It has been my experience that the background you've described is caused by
the static that's 'built up' by wiping around the circular hyrdrophobic
barrier after rinsing. As other HistoNetters have stated, this can be avoided by
applying hyrdrophobic barriers that run the width of the slide, and removing
excess buffer by simply tilting the edge of the slide onto an absorbent
surface (rather that wiping).
Date: Fri, 9 May 2008 16:17:40 -0700 (PDT)
From: Karla Arrington
Subject: [Histonet] IHC Background Staining
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I do Immuno's by hand and I am getting background DAB precipitate on the
inside circle of my sections.
What is causing this and how do I get rid of it?
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