RE: [Histonet] IHC Background Staining

It has been my experience that the background you've described is  caused by 
the static that's 'built up' by wiping around the circular  hyrdrophobic 
barrier after rinsing.  As other HistoNetters  have stated, this can be avoided by 
applying hyrdrophobic barriers  that run the width of the slide, and removing 
excess buffer by simply  tilting the edge of the slide onto an absorbent 
surface (rather that  wiping).

Message: 8
Date: Fri, 9 May 2008  16:17:40 -0700 (PDT)
From: Karla Arrington  
Subject: [Histonet] IHC Background  Staining
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I do Immuno's by hand and I am getting background DAB  precipitate on the 
inside circle of my sections. 
What is causing this and  how do I get rid of it?

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