The most common error made hand staining and the easiest to resolve is
inadequate washing between steps. Make sure reagent is thoroughly
drained from the slide and rinse with copious amts of buffer before
proceeding to the next step
Rena 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Karla
Arrington
Sent: Friday, May 09, 2008 7:18 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] IHC Background Staining

I do Immuno's by hand and I am getting background DAB precipitate on the
inside circle of my sections. 
What is causing this and how do I get rid of it?


 
________________________________________________________________________
____________
Be a better friend, newshound, and 
know-it-all with Yahoo! Mobile.  Try it now.
http://mobile.yahoo.com/;_ylt=Ahu06i62sR8HDtDypao8Wcj9tAcJ
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet