RE: [Histonet] IHC Background Staining

From:"Rena Fail"

The most common error made hand staining and the easiest to resolve is
inadequate washing between steps. Make sure reagent is thoroughly
drained from the slide and rinse with copious amts of buffer before
proceeding to the next step

-----Original Message-----
[] On Behalf Of Karla
Sent: Friday, May 09, 2008 7:18 PM
Subject: [Histonet] IHC Background Staining

I do Immuno's by hand and I am getting background DAB precipitate on the
inside circle of my sections. 
What is causing this and how do I get rid of it?

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