RE: [Histonet] AEC chromogen - coverslipping

From:sheila adey

We were of the understanding that we could just use the aqueous mountant with the cover glass in the over and they were good to go?Sheila Adey HT MLTPort Huron HospitalMichigan> From:> To:;> Date: Fri, 16 May 2008 13:18:31 -0600> Subject: Re: [Histonet] AEC chromogen - coverslipping> CC: > > Richard,> > We have used the liquid mounting media, Crystal Mount from Biomeda - > ThermoFisher used to carry this. Do the liquid coverslip first, let it dry > according to directions i.e. several ways with heat, then mount a permanent > coverglass over the top of this. AEC is preserved.> > If you try to look at the section before mounting a permanent coverslip, > it can look a bit out of focus. Make sure the media covers the section > smoothly and as flat at possible to avoid the "waves" of media.> > Someone recently posted the contact for Biomeda on Histonet on the chance > ThermoFisher is not selling Crystal Mount anymore. There are other > companies that supply this type of mounting media but not sure which > vendors.> > Good luck> > Gayle M. Callis> HTL/HT/MT(ASCP)> Bozeman MT 59715> > ----- Original Message ----- > From: "Richard Cartun" > To: "Histonet" > Sent: Friday, May 16, 2008 11:22 AM> Subject: [Histonet] AEC chromogen - coverslipping> > > If you use AEC as a chromogen for IHC staining, what are using to protect it > before putting the slides into xylol for permanent coverslipping?> > Thanks!> > Richard> > Richard W. Cartun, Ph.D.> Director, Immunopathology & Histology> Assistant Director, Anatomic Pathology> Hartford Hospital> 80 Seymour Street> Hartford, CT 06102> (860) 545-1596> (860) 545-0174 Fax> > Confidentiality Notice> > This e-mail message, including any attachments, is for the sole use of the > intended recipient(s) and may contain confidential or proprietary > information which is legally privileged. Any unauthorized review, use, > disclosure, or distribution is prohibited. If you are not the intended > recipient, please promptly contact the sender by reply e-mail and destroy > all copies of the original message.> > _______________________________________________> Histonet mailing list>> > > > _______________________________________________> Histonet mailing list>>

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