Does anyone know why CaCl2 is used in trypsin buffers for retrieval of
antigen in paraformaldehyde-fixed, paraffin-embedded tissue sections? Is
it really necessary? Can I just use a Trypsin-EDTA solution we already
have in the lab?
Merced M Leiker
Research Technician II
354 BRB (Lee Lab) / 140 Farber Hall (mail)
School of Medicine and Biomedical Sciences
State University of New York at Buffalo
3435 Main St, Buffalo, NY 14214
Ph: (716) 829-6033
Fx: (716) 829-2725
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