Hi everyone, I`m looking for a protocol for microwave deparaffinizing
, to eliminate the use of xylene, does any have one to submit, tanks
from here in southamerica.
Regards, Sebastian , histotechnology student, EUTM, fmed , UDELAR.
Montevideo, Uruguay.
2008/5/17, histonet-request@lists.utsouthwestern.edu
:
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> Today's Topics:
>
> 1. Re: Keratin-903 on Liver (Patti Loykasek)
> 2. AEC chromogen - coverslipping (Richard Cartun)
> 3. Sakura tape coverslip problem (CHRISTIE GOWAN)
> 4. RE: fresh-frozen mouse tail sectioning
> (Rachel, Rivka (NIH/NEI) [E])
> 5. Calcium oxylate stain (Mary Lloyd)
> 6. unsubscribe (Danielle Crippen)
> 7. Re: Sakura tape coverslip problem (tissuetech@juno.com)
> 8. RE: Calcium oxylate stain (Thomas Jasper)
> 9. Re: Calcium oxylate stain (Gayle Callis)
> 10. AEC Chromagen - Coverslipping (Paula Pierce)
> 11. Re: AEC chromogen - coverslipping (Gayle Callis)
> 12. looking for c-kit (Merced Leiker)
> 13. Re: AEC chromogen - coverslipping (Rene J Buesa)
> 14. Re: Sakura tape coverslip problem (Rene J Buesa)
> 15. RE: AEC chromogen - coverslipping (Linke_Noelle)
> 16. WAP ab (Margaryan, Naira)
> 17. Re: Hematoxylin Shortages (Shelly Coker)
> 18. Histoscreen Cassettes (Shelly Coker)
> 19. Microwave tissue processor (San Tin)
> 20. Re: Histoscreen Cassettes (Shelly Coker)
> 21. Re: Microwave tissue processor (Rene J Buesa)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Fri, 16 May 2008 10:22:21 -0700
> From: Patti Loykasek
> Subject: Re: [Histonet] Keratin-903 on Liver
> To: "Metzger, Kenneth" ,
>
> Message-ID:
> Content-Type: text/plain; charset="US-ASCII"
>
> Hi Ken. By referring to K-903, I believe you mean cytokeratin clone 34BE12.
> The CK-903 designation is actually an old catalog number, not the name of
> the keratin. Dr. Allen Gown made the 34BE12 antibody about 20 years ago.
> It's a high molecular weight keratin (CK1,5,10 & 14). Just a bit of history
> for you. But on to helping with your staining issues. This keratin should
> stain the bile ducts in liver. What pretreatment are you using? This
> antibody usually works with either an enzyme digestion or a gentle heat
> retrieval in citrate. Let me know if you need more info.
>
>
> Patti Loykasek BS, HTL, QIHC
> PhenoPath Laboratories
> Seattle, WA
>
>
>
>
>
>> One of our pathologists wants us to use K-903 on liver to tag the bile
>> duct.
>> Though our procedure works great on prostate we are having trouble with it
>> on
>> the liver..any suggestions? Thanks
>>
>> Ken Metzger HTL(ASCP)
>> Histology Supervisor
>> ARUP Laboratories
>> 500 Chipeta way
>> Salt Lake City, UT 84108
>> 801.583.2787 ext 3101
>>
>>
>> - ------------------------------------------------------------------
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>
>
>
> This e-mail message, including any attachments, is for the sole use of the
> intended recipients and may contain privileged information. Any unauthorized
> review, use, disclosure or distribution is prohibited. If you are not the
> intended
> recipient, please contact the sender by e-mail and destroy all copies of the
> original message, or you may call PhenoPath Laboratories, Seattle, WA U.S.A.
> at (206) 374-9000.
>
>
>
>
> ------------------------------
>
> Message: 2
> Date: Fri, 16 May 2008 13:22:35 -0400
> From: "Richard Cartun"
> Subject: [Histonet] AEC chromogen - coverslipping
> To: "Histonet"
> Message-ID: <482D8A9B020000770000C9EC@gwmail4.harthosp.org>
> Content-Type: text/plain; charset=US-ASCII
>
> If you use AEC as a chromogen for IHC staining, what are using to protect it
> before putting the slides into xylol for permanent coverslipping?
>
> Thanks!
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Immunopathology & Histology
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596
> (860) 545-0174 Fax
>
> Confidentiality Notice
>
> This e-mail message, including any attachments, is for the sole use of the
> intended recipient(s) and may contain confidential or proprietary
> information which is legally privileged. Any unauthorized review, use,
> disclosure, or distribution is prohibited. If you are not the intended
> recipient, please promptly contact the sender by reply e-mail and destroy
> all copies of the original message.
>
>
>
> ------------------------------
>
> Message: 3
> Date: Fri, 16 May 2008 17:24:32 +0000
> From: "CHRISTIE GOWAN"
> Subject: [Histonet] Sakura tape coverslip problem
> To: histonet@lists.utsouthwestern.edu
> Message-ID:
> Content-Type: text/plain; format=flowed
>
>
> Does anyone have a recovery method for tape coverslips that become detatched
> from the slide taking the tissue with it? I have tried several things but
> have not found a really good way to do this. I called the company and they
> did not have a procedure.
> Thanks,
> Christie Gowan
> UAB Hospital
> Birmingham, AL
>
>
>
>
>
> ------------------------------
>
> Message: 4
> Date: Fri, 16 May 2008 13:56:06 -0400
> From: "Rachel, Rivka (NIH/NEI) [E]"
> Subject: RE: [Histonet] fresh-frozen mouse tail sectioning
> To: "Gaupp, Dina D " ,
>
> Message-ID:
>
> Content-Type: text/plain; charset="iso-8859-1"
>
> Do you have to freeze or section the tissue at all? If there is significant
> beta-galactosidase activity, you should be able to just put the tail tip in
> X-gal and see if it turns blue, having of course appropriate positive and
> negative control tails that are known to carry or not carry, respectively,
> the relevant transgene.
>
> Rivka
>
>
> --
> Rivka A. Rachel, MD, PhD
> Staff Scientist, National Eye Institute
> Neurobiology-Neurodegeneration and Repair Laboratory
> Tel: 301 443-4906
>
>
>
> -----Original Message-----
> From: Gaupp, Dina D [mailto:dgaupp@tulane.edu]
> Sent: Fri 5/16/2008 12:04 PM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] fresh-frozen mouse tail sectioning
>
> To Whomever This Applies:
>
> I am having big problems sectioning mouse tail, approximately 2mm in
> thickness, vertical embedded in OCT for frozen sections. The tissue is
> fresh-frozen because the principle investigator would like to detect an
> enzyme x-gal. No fix, no cryopreservation - the tissue was not immersed in
> any type of solution. He snipped the ends of a mouse tail & immediately
> gave me the tissues. I embedded the tissue vertically in OCT & flash froze
> at -80C. Upon sectioning, the tissue rolled. I could not for the life of
> me get 1 section. All the tissues rolled & its so tiny to begin with that
> it was hard for me to grab it. I used the anti-roll plate, hoping it would
> hold the tissue in place but it still rolled. I changed temperature
> settings(increase & decrease temp), thickness, angle, rubbed it with my
> fingers. Everything I could think of to get a section. I asked someone
> else in the lab to try & they couldn't get a section. It was like the
> tissue completely separated from OCT. The principle investigator will give
> me more samples & I don't want this to happen again.
>
> Can anyone in histoland help me, tell me what I didn't do or what I did
> wrong?
>
> Dina D. Gaupp, BS, MT
> Senior Lab Supervisor
> Center for Gene Therapy, SL-99
> Tulane University Health Science Center
> 1430 Tulane Ave
> New Orleans, La 70112
> Lab: 504-988-1194
> dgaupp@tulane.edu
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 5
> Date: Fri, 16 May 2008 14:04:41 -0400
> From: "Mary Lloyd"
> Subject: [Histonet] Calcium oxylate stain
> To:
> Message-ID:
> <2096B6FC591D034FA50AFFD7A42EE96506774F61@dental.dentnet.dent.ohio-state.edu>
>
> Content-Type: text/plain; charset="us-ascii"
>
> My oral pathologist is requesting a specific stain for calcium oxylate.
> I have done von kossa for calcium but he wants something more specific.
> I would appreciate any help. Thanks Mary
>
>
> ------------------------------
>
> Message: 6
> Date: Fri, 16 May 2008 11:07:51 -0700
> From: "Danielle Crippen"
> Subject: [Histonet] unsubscribe
> To:
> Message-ID:
>
> Content-Type: text/plain; charset="us-ascii"
>
>
>
>
>
> Danielle Crippen
>
> Morphology and Imaging Core
>
> x2046
>
>
>
>
>
> ------------------------------
>
> Message: 7
> Date: Fri, 16 May 2008 18:28:10 GMT
> From: "tissuetech@juno.com"
> Subject: Re: [Histonet] Sakura tape coverslip problem
> To: christiegowan@msn.com
> Cc: histonet@lists.utsouthwestern.edu
> Message-ID: <20080516.132810.15454.0@webmail14.vgs.untd.com>
> Content-Type: text/plain; charset=windows-1252
>
> Christie
> Sorry about your problem. At this time there is nothing that I know of
> where you can remove the tissue from the tape and recover slip. I know you
> are caught between a rock & a hard place, but over the years no one that I
> know has ever been able to retrieve and remount. Sorry I can't be of more
> help.
> Fred S - Tissue Techniques Path Labs
>
> _____________________________________________________________
> Click here to save cash and find low rates on auto loans.
> http://thirdpartyoffers.juno.com/TGL2121/fc/Ioyw6i3ndyH35dbInkZRPhRa58lNrdsPTYWRhADbbiHInlQ8K8YNeR/?count=1234567890
>
>
> ------------------------------
>
> Message: 8
> Date: Fri, 16 May 2008 11:41:27 -0700
> From: "Thomas Jasper"
> Subject: RE: [Histonet] Calcium oxylate stain
> To: "Mary Lloyd"
> Cc: histonet@lists.utsouthwestern.edu
> Message-ID:
> <90354A475B420441B2A0396E5008D4965E20BF@copc-sbs.COPC.local>
> Content-Type: text/plain; charset="US-ASCII"
>
> How about Alizarin Red S?
> Tom J.
>
> Thomas Jasper HT (ASCP) BAS
> Histology Supervisor
> Central Oregon Regional Pathology Services
> Bend, Oregon 97701
> 541/693-2677
> tjasper@copc.net
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Mary
> Lloyd
> Sent: Friday, May 16, 2008 11:05 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Calcium oxylate stain
>
> My oral pathologist is requesting a specific stain for calcium oxylate.
> I have done von kossa for calcium but he wants something more specific.
> I would appreciate any help. Thanks Mary
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ------------------------------
>
> Message: 9
> Date: Fri, 16 May 2008 13:10:02 -0600
> From: "Gayle Callis"
> Subject: Re: [Histonet] Calcium oxylate stain
> To: "Mary Lloyd" ,
>
> Message-ID: <006401c8b788$71b60a50$6501a8c0@DHXTS541>
> Content-Type: text/plain; format=flowed; charset="iso-8859-1";
> reply-type=original
>
> Pizzolata's stain for calcium oxalate. I has been discussed on Histonet in
> the past, and you may be able to pick up the method on the web. Sheehan and
> Hrapchak Theory and Practice of Histotechnology, second edition has the
> method. Very easy to do. We ran the von Kossa on an adjacent section to
> distinguish between calcium oxalate and other calcium salt deposits, and
> eliminate one or the other for diagnostic purposes.
>
> A good calcium oxalate positive control is a dog or cat kidney that drank
> antifreeze containing ethylene glycol. The crystals are a bit refractile
> looking in an H&E section. A local veterinary diagnositic laboratory may
> have tissues available.
>
> Gayle M. Callis
> HTL/HT/MT(ASCP)
> Bozeman MT 59715
>
>
> ----- Original Message -----
> From: "Mary Lloyd"
> To:
> Sent: Friday, May 16, 2008 12:04 PM
> Subject: [Histonet] Calcium oxylate stain
>
>
> My oral pathologist is requesting a specific stain for calcium oxylate.
> I have done von kossa for calcium but he wants something more specific.
> I would appreciate any help. Thanks Mary
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 10
> Date: Fri, 16 May 2008 12:12:53 -0700 (PDT)
> From: Paula Pierce
> Subject: [Histonet] AEC Chromagen - Coverslipping
> To: Histonet
> Message-ID: <150685.15173.qm@web50109.mail.re2.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> I use Crystal Mount. After rinsing to blue the hematoxylin, place a drop to
> cover the section, drain excess, let dry completely, and coverslip with
> resin mounting media.
> Paula Pierce, HTL(ASCP)HT
>
> Excalibur Pathology, Inc.
> 631 N. Broadway Ave.
> Moore, OK 73160
> 405-570-6679 cell
> 405-759-3953 lab
> contact@excaliburpathology.com
> www.excaliburpathology.com
>
> ------------------------------
>
> Message: 11
> Date: Fri, 16 May 2008 13:18:31 -0600
> From: "Gayle Callis"
> Subject: Re: [Histonet] AEC chromogen - coverslipping
> To: "Richard Cartun" , "Histonet"
>
> Message-ID: <006e01c8b789$a0f962c0$6501a8c0@DHXTS541>
> Content-Type: text/plain; format=flowed; charset="iso-8859-1";
> reply-type=original
>
> Richard,
>
> We have used the liquid mounting media, Crystal Mount from Biomeda -
> ThermoFisher used to carry this. Do the liquid coverslip first, let it dry
> according to directions i.e. several ways with heat, then mount a permanent
> coverglass over the top of this. AEC is preserved.
>
> If you try to look at the section before mounting a permanent coverslip,
> it can look a bit out of focus. Make sure the media covers the section
> smoothly and as flat at possible to avoid the "waves" of media.
>
> Someone recently posted the contact for Biomeda on Histonet on the chance
> ThermoFisher is not selling Crystal Mount anymore. There are other
> companies that supply this type of mounting media but not sure which
> vendors.
>
> Good luck
>
> Gayle M. Callis
> HTL/HT/MT(ASCP)
> Bozeman MT 59715
>
> ----- Original Message -----
> From: "Richard Cartun"
> To: "Histonet"
> Sent: Friday, May 16, 2008 11:22 AM
> Subject: [Histonet] AEC chromogen - coverslipping
>
>
> If you use AEC as a chromogen for IHC staining, what are using to protect it
> before putting the slides into xylol for permanent coverslipping?
>
> Thanks!
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Immunopathology & Histology
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596
> (860) 545-0174 Fax
>
> Confidentiality Notice
>
> This e-mail message, including any attachments, is for the sole use of the
> intended recipient(s) and may contain confidential or proprietary
> information which is legally privileged. Any unauthorized review, use,
> disclosure, or distribution is prohibited. If you are not the intended
> recipient, please promptly contact the sender by reply e-mail and destroy
> all copies of the original message.
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 12
> Date: Fri, 16 May 2008 15:40:16 -0400
> From: Merced Leiker
> Subject: [Histonet] looking for c-kit
> To: histonet@lists.utsouthwestern.edu
> Cc: histonet@lists.utsouthwestern.edu
> Message-ID: <541B786D4309163175E035B6@bchwxp2702.ad.med.buffalo.edu>
> Content-Type: text/plain; charset=us-ascii; format=flowed
>
> I'm looking for an indirect immunofluorescence procedure for localizing
> c-kit on formaldehyde-fixed, paraffin-embedded tissue.
>
> What type of AR do you use, and do you permeabilize?
>
> Thanks,
>
> Merced M Leiker
> Research Technician II
> 354 BRB (Lee Lab) / 140 Farber Hall (mail)
> School of Medicine and Biomedical Sciences
> State University of New York at Buffalo
> 3435 Main St, Buffalo, NY 14214
> Ph: (716) 829-6033
> Fx: (716) 829-2725
>
>
>
>
> ------------------------------
>
> Message: 13
> Date: Fri, 16 May 2008 13:03:50 -0700 (PDT)
> From: Rene J Buesa
> Subject: Re: [Histonet] AEC chromogen - coverslipping
> To: Richard Cartun , Histonet
>
> Message-ID: <706741.94015.qm@web65709.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> I don't think you can do much, except of using a water soluble mounting
> medium able to solidify permanently.
> René J.
>
> Richard Cartun wrote:
> If you use AEC as a chromogen for IHC staining, what are using to protect
> it before putting the slides into xylol for permanent coverslipping?
>
> Thanks!
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Immunopathology & Histology
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596
> (860) 545-0174 Fax
>
> Confidentiality Notice
>
> This e-mail message, including any attachments, is for the sole use of the
> intended recipient(s) and may contain confidential or proprietary
> information which is legally privileged. Any unauthorized review, use,
> disclosure, or distribution is prohibited. If you are not the intended
> recipient, please promptly contact the sender by reply e-mail and destroy
> all copies of the original message.
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> Message: 14
> Date: Fri, 16 May 2008 13:08:07 -0700 (PDT)
> From: Rene J Buesa
> Subject: Re: [Histonet] Sakura tape coverslip problem
> To: CHRISTIE GOWAN ,
> histonet@lists.utsouthwestern.edu
> Message-ID: <897976.53776.qm@web65704.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Christie:
> Put the tape with the section back over the slide. Place a glass coverslip
> on top held with a paper clip. Immerse the whole thing in acetone. Hopefully
> the plastic will dissolve leaving the tissue between the glass coverslip and
> the slide. Eliminate the acetone by capillarity with xylene and in the same
> way add the coverslipping medium. Work as gentle as you can. I have done it!
> René J.
>
> CHRISTIE GOWAN wrote:
>
>
>
>
> ------------------------------
>
> Message: 15
> Date: Fri, 16 May 2008 13:09:59 -0700
> From: "Linke_Noelle"
> Subject: RE: [Histonet] AEC chromogen - coverslipping
> To: "Gayle Callis" , "Richard Cartun"
> , "Histonet"
> Message-ID:
> <5C3DA4BE34AA0641BAA10A7C1478B60527D26C@IRMAIL132.irvine.allergan.com>
> Content-Type: text/plain; charset="iso-8859-1"
>
> I use BioCare's Romulin AEC.....xylene compatible, it's beautiful stuff!!
>
> Noelle
>
> Noëlle Linke, MS, HTL(ASCP)QIHC
> Allergan, Inc
> 2525 Dupont Drive RD-2A
> Irvine, CA 92612
> 714-246-5568
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis
> Sent: Friday, May 16, 2008 12:19 PM
> To: Richard Cartun; Histonet
> Subject: Re: [Histonet] AEC chromogen - coverslipping
>
> Richard,
>
> We have used the liquid mounting media, Crystal Mount from Biomeda -
> ThermoFisher used to carry this. Do the liquid coverslip first, let it dry
> according to directions i.e. several ways with heat, then mount a permanent
> coverglass over the top of this. AEC is preserved.
>
> If you try to look at the section before mounting a permanent coverslip,
> it can look a bit out of focus. Make sure the media covers the section
> smoothly and as flat at possible to avoid the "waves" of media.
>
> Someone recently posted the contact for Biomeda on Histonet on the chance
> ThermoFisher is not selling Crystal Mount anymore. There are other
> companies that supply this type of mounting media but not sure which
> vendors.
>
> Good luck
>
> Gayle M. Callis
> HTL/HT/MT(ASCP)
> Bozeman MT 59715
>
> ----- Original Message -----
> From: "Richard Cartun"
> To: "Histonet"
> Sent: Friday, May 16, 2008 11:22 AM
> Subject: [Histonet] AEC chromogen - coverslipping
>
>
> If you use AEC as a chromogen for IHC staining, what are using to protect it
> before putting the slides into xylol for permanent coverslipping?
>
> Thanks!
>
> Richard
>
> Richard W. Cartun, Ph.D.
> Director, Immunopathology & Histology
> Assistant Director, Anatomic Pathology
> Hartford Hospital
> 80 Seymour Street
> Hartford, CT 06102
> (860) 545-1596
> (860) 545-0174 Fax
>
> Confidentiality Notice
>
> This e-mail message, including any attachments, is for the sole use of the
> intended recipient(s) and may contain confidential or proprietary
> information which is legally privileged. Any unauthorized review, use,
> disclosure, or distribution is prohibited. If you are not the intended
> recipient, please promptly contact the sender by reply e-mail and destroy
> all copies of the original message.
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 16
> Date: Fri, 16 May 2008 15:41:47 -0500
> From: "Margaryan, Naira"
> Subject: [Histonet] WAP ab
> To:
> Message-ID:
>
>
> Content-Type: text/plain; charset="us-ascii"
>
> HI Dears,
>
>
>
> It is late of Friday and time to go home.........
>
>
> But I need to find good WAP Ab for immuno. It will be appreciated if
> anybody could suggest the WAP Ab for immuno and what kind of Retrieval
> to use.
>
>
> Have a nice weekend,
>
> Naira
>
>
>
> Naira V. Margaryan, D.V.M., Ph.D.
>
> Research Scientist
>
> Children's Memorial Research Center
>
> 2300 Children's Plaza, Box 222
>
> Chicago, IL 60614-3363
>
> Tel: 773-755-6340
>
> Fax: 773-755-6594
>
> nmargaryan@childrensmemorial.org
>
>
>
>
> For Express Mail:
>
> CMRC, Room C.473
>
> 2430 N. Halsted Street
>
> Chicago, IL 60614-4314
>
>
>
>
>
> ------------------------------
>
> Message: 17
> Date: Fri, 16 May 2008 15:17:28 -0700 (PDT)
> From: Shelly Coker
> Subject: [Histonet] Re: Hematoxylin Shortages
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <345606.59906.qm@web90303.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> I just had a demo of the Surgipath staining system, and the rep informed me
> that there is going to be a shortage, and as of right now, Surgipath will
> not be shipping their Hematoxylin to new customers, only those currently
> using the staining system. He did inform me that they anticipated being
> able to send this product out to new cutomers in August, based on
> information received from the production side. I would take that to imply
> that they plan to have this under control by August, or at least that's what
> I am hoping!
>
> Regards,
>
> Michelle Coker
> HT(ASCP)cm
>
>
>
> ------------------------------
>
> Message: 18
> Date: Fri, 16 May 2008 15:23:53 -0700 (PDT)
> From: Shelly Coker
> Subject: [Histonet] Histoscreen Cassettes
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <163239.63792.qm@web90303.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Hi everyone!
>
> I have some Histoscreen cassettes I would like to dispose of. These
> cassettes have been in our storage for close to a year. The colors I have
> are blue and lilac. We currently process our specimens in a microwave
> processor, and these cassettes wreak havoc on our tissue. If there is
> anyone interested, please contact me off-list.
>
> Thanks,
>
> Michelle Coker
> HT(ASCP)cm
>
>
>
> ------------------------------
>
> Message: 19
> Date: Fri, 16 May 2008 20:30:35 -0700 (PDT)
> From: San Tin
> Subject: [Histonet] Microwave tissue processor
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <309011.60354.qm@web55806.mail.re3.yahoo.com>
> Content-Type: text/plain; charset="us-ascii"
>
>
> Dear All,
>
> Our hospital is looking for a microwave rapid tissue processor. Any
> comments on any instruments aspecially Sakura Tissue TeK Xpress? Any
> finding with Immunohistochemistry?
>
> Please Give comments.
>
>
> Regards,
>
> San
>
>
> ------------------------------
>
> Message: 20
> Date: Fri, 16 May 2008 20:43:41 -0700 (PDT)
> From: Shelly Coker
> Subject: [Histonet] Re: Histoscreen Cassettes
> To: histonet@lists.utsouthwestern.edu
> Message-ID: <479141.33606.qm@web90305.mail.mud.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> I have found a taker for the cassettes. Have a great weekend everyone!
>
>
>
>
>
> ------------------------------
>
> Message: 21
> Date: Sat, 17 May 2008 07:21:31 -0700 (PDT)
> From: Rene J Buesa
> Subject: Re: [Histonet] Microwave tissue processor
> To: san.htin@yahoo.com, histonet@lists.utsouthwestern.edu
> Message-ID: <677561.10453.qm@web65709.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Sakura Tissue Tek Xpress (the first version with 4 ovens, or the newer with
> only two) is a good instrument although "somewhat" pricey ($250,000 for the
> first and half that amount for the second).
> There are other less expensive alternatives that allow also a "Lean" like
> work flow.
> I am sending you an article on the subject.
> René J.
>
> San Tin wrote:
>
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
> ------------------------------
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> End of Histonet Digest, Vol 54, Issue 25
> ****************************************
>
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