CaCl makes the trypsin "work" chelating (?spelling) agent from what I=20
recall. You can buy ready to use tablets from Sigma T 7168 just
dissolve in 1cc DI water 40' 20 min.
histonet-request@lists.utsouthwestern.edu wrote:
> Send Histonet mailing list submissions to
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> Today's Topics:
>
> 1. RE: Bone Flap from Skull (Sebree Linda A.)
> 2. Re: Bone Flap from Skull (Rene J Buesa)
> 3. Re: Bone Flap from Skull (Geoff McAuliffe)
> 4. Re: Bone Flap from Skull (Rivka Rachel)
> 5. California licensure (Martin, Erin)
> 6. (no subject) (Kathleen Boozer)
> 7. CAP Her2Neu regs (Satterfield, Marirose)
> 8. Why use Calcium Chloride in Trypsin Antigen Retrieval?
> (Merced Leiker)
> 9. RE: Polymer IHC Detection (Wynn, Carmen)
> 10. ERRATUM in Why use Calcium Chloride in Trypsin Antigen
> Retrieval? (Merced Leiker)
> 11. Bone Marrow Cores (Metzger, Kenneth)
> 12. pigment in bone marrow clot sections (Mike Pence)
> 13. Epithelial specific antigen EP CAM (AnitaIBSC)
> 14. RE: pigment in bone marrow clot sections (Liz Chlipala)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Thu, 8 May 2008 12:02:34 -0500
> From: "Sebree Linda A."
> Subject: RE: [Histonet] Bone Flap from Skull
> To: "Lott, Robert" ,
>
> Message-ID:
>
>
> Content-Type: text/plain; charset="us-ascii"
>
> Just off the top of my head (no pun intended) I would think an organ
> preservation solution might be reasonable assuming that your institution
> is involved in transplantation.
>
> Linda Sebree, HT(ASCP)
> University of Wisconsin Hospital & Clinics
> IHC/ISH Laboratory
> A4/204-3224
> 600 Highland Ave.
> Madison, WI 53792
> (608)265-6596
> FAX: (608)262-7174
>
>
> -----Original Message-----
> From: histonet-bounces@lists.utsouthwestern.edu
> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott,
> Robert
> Sent: Thursday, May 08, 2008 11:58 AM
> To: histonet@lists.utsouthwestern.edu
> Subject: [Histonet] Bone Flap from Skull
>
>
> A neurosurgeon just brought a bone flap from a patient and wants to
> replace it 3-4 days from now...
>
> Do any of you have suggestions or recommendations for storing the bone
> flap in the lab until he needs it again???
>
>
>
> Need help fast!
>
>
>
> Thanks!
>
>
>
>
>
> Robert L. Lott, HTL(ASCP)
>
> Manager, Anatomic Pathology
>
> Trinity Medical Center / LabFirst
>
> 800 Montclair Road
>
> Birmingham, AL 35213
>
> 205-592-5388
>
> 205-592-5646 - fax
>
> robert.lott@triadhospitals.com
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ------------------------------
>
> Message: 2
> Date: Thu, 8 May 2008 10:15:14 -0700 (PDT)
> From: Rene J Buesa
> Subject: Re: [Histonet] Bone Flap from Skull
> To: "Lott, Robert" ,
> histonet@lists.utsouthwestern.edu
> Message-ID: <160753.70968.qm@web65705.mail.ac4.yahoo.com>
> Content-Type: text/plain; charset=iso-8859-1
>
> Freeze it at -80ºC
> René J.
>
> "Lott, Robert" wrote:
> A neurosurgeon just brought a bone flap from a patient and wants to
> replace it 3-4 days from now...
>
> Do any of you have suggestions or recommendations for storing the bone
> flap in the lab until he needs it again???
>
>
>
> Need help fast!
>
>
>
> Thanks!
>
>
>
>
>
> Robert L. Lott, HTL(ASCP)
>
> Manager, Anatomic Pathology
>
> Trinity Medical Center / LabFirst
>
> 800 Montclair Road
>
> Birmingham, AL 35213
>
> 205-592-5388
>
> 205-592-5646 - fax
>
> robert.lott@triadhospitals.com
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
> ---------------------------------
> Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it now.
>
> ------------------------------
>
> Message: 3
> Date: Thu, 08 May 2008 13:16:51 -0400
> From: Geoff McAuliffe
> Subject: Re: [Histonet] Bone Flap from Skull
> Cc: histonet@lists.utsouthwestern.edu, "Lott, Robert"
>
> Message-ID: <48233583.3050809@umdnj.edu>
> Content-Type: text/plain; charset=ISO-8859-1; format=flowed
>
> You do not need help with this, you need to get far away from this.
> I can't believe your institution does not have a protocol for this!
> Are you really going to take recommendations from Histoneters who are
> not MDs?
> I can just see you testifying at the malpractice trail (after the=20
> infected/necrotic tissue causes serious
> illness or death) that someone on histonet told you it was OK to ...........
> Get my drift?
> This is obvious malpractice on the surgeon's part. Run like hell.
>
> Geoff
>
>> -----Original Message-----
>> From: histonet-bounces@lists.utsouthwestern.edu
>> [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Lott,
>> Robert
>> Sent: Thursday, May 08, 2008 11:58 AM
>> To: histonet@lists.utsouthwestern.edu
>> Subject: [Histonet] Bone Flap from Skull
>>
>>
>> A neurosurgeon just brought a bone flap from a patient and wants to
>> replace it 3-4 days from now...
>>
>> Do any of you have suggestions or recommendations for storing the bone
>> flap in the lab until he needs it again???
>>
>>
>>
>> Need help fast!
>>
>>
>>
>> Thanks!
>>
>>
>>
>>
>>
>> Robert L. Lott, HTL(ASCP)
>>
>> Manager, Anatomic Pathology
>>
>> Trinity Medical Center / LabFirst
>>
>> 800 Montclair Road
>>
>> Birmingham, AL 35213
>>
>> 205-592-5388
>>
>> 205-592-5646 - fax
>>
>> robert.lott@triadhospitals.com
>>
>>
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>> _______________________________________________
>> Histonet mailing list
>> Histonet@lists.utsouthwestern.edu
>> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>>
>>
>>
>>
>
>
>
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