OK, I've been working on this for over a week and finally decided I need
help. LS174T tumor cells (a human colon cancer cell line) were implanted
into mouse lymph nodes. I'm trying to immuno-stain these implanted cells.
I tried a pan cytokeratin antibody and a CK20 cytokeratin antibody and
neither worked. Has anyone here ever worked with this cell line? Any
suggestions on how to differentiate these cells from the normal mouse cells?
Thanks!
Jim
_____________________
Jim Staruk
Mass Histology Service
www.masshistology.com
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