[Histonet] 4% paraformaldehyde all over again

From:"Downs, Heather M."




 We purchase paraformaldehyde in powder form, from Fisher, and make our own 4%
paraformaldehyde.  We use it for perfusion, of animals and making PLP for our
biopsies.

Heather

-----Original Message-----
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Sent: Tuesday, May 13, 2008 1:03 PM
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Subject: Histonet Digest, Vol 54, Issue 17

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Today's Topics:

   1. C =Con(with) S means Sans(without)-Latin origin (Madary, Joseph)
   2. RE: C =Con(with) S means Sans(without)-Latin origin
      (Bonner, Janet)
   3. ICC problem (Karla Arrington)
   4. Bone Marrow Staining (Karla Arrington)
   5. Re: Bone Marrow Staining (Rene J Buesa)
   6. Re: IHC Background Staining (Victoria Baker)
   7. non specific staining (Teisha Robertson)
   8. RE: RE: Latin - Thank goodness! (Ingles Claire)
   9. RE: RE: [Histonet] I need more ammunition (Thomas Jasper)
  10. Method validation (Oto, Carrie)
  11. Re: C =Con(with) S means Sans(without)-Latin origin (Joe Nocito)
  12. Re: 4% paraformaldehyde all over again (John Kiernan)
  13. thanks (louise renton)
  14. RE: 4% paraformaldehyde all over again (Smith, Allen)
  15. Catalase Staining for Liver Aspirates Help Please (Amy Porter)
  16. Amended reports- Cerner CoPath users (Angela Bitting)
  17. Standardized Microtomes (Nancy Schmitt)
  18. Apipophilin and MCM (Michele Wich)
  19. rubber stoppers & tubing (Atoska Gentry)
  20. RE: Standardized Microtomes (Liz Chlipala)
  21. Re: Standardized Microtomes (Jackie M O'Connor)
  22. Paraffin thickness standard or measurement? (amy rizzo)
  23. RE: Standardized Microtomes (Joyce Cline)


----------------------------------------------------------------------

Message: 1
Date: Mon, 12 May 2008 13:08:59 -0400
From: "Madary, Joseph" 
Subject: [Histonet] C =Con(with) S means Sans(without)-Latin origin
To: 
Message-ID:
	
Content-Type: text/plain;	charset="us-ascii"



Nick Madary, HT/HTL(ASCP)QIHC
Medimmune Histology Laboratory
One Medimmune Way
Gaithersburg, MD 20878

ph 301.398.4745/6360
fx  301.398.9745

To the extent this electronic communication or any of its attachments
contain information that is not in the public domain, such information
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expected to be used only by the individual(s) for whom it is intended.
If you have received this electronic communication in error, please
reply to the sender advising of the error in transmission and delete the
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purpose. Thank you for your cooperation.





To the extent this electronic communication or any of its attachments contain
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MedImmune to be confidential and proprietary.  This communication is expected to
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------------------------------

Message: 2
Date: Mon, 12 May 2008 13:19:14 -0400
From: "Bonner, Janet" 
Subject: RE: [Histonet] C =Con(with) S means Sans(without)-Latin
	origin
To: "Madary, Joseph" ,
	histonet@lists.utsouthwestern.edu
Message-ID:
	<5F31F38C96781A4FBE3196EBC22D47807F2647@fhosxchmb006.ADVENTISTCORP.NET>
	
Content-Type: text/plain; charset=iso-8859-1

OK, I use w/ = with, and w/o = without.  -American

________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Madary, Joseph
Sent: Mon 5/12/2008 1:08 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] C =Con(with) S means Sans(without)-Latin origin





Nick Madary, HT/HTL(ASCP)QIHC
Medimmune Histology Laboratory
One Medimmune Way
Gaithersburg, MD 20878

ph 301.398.4745/6360
fx  301.398.9745

To the extent this electronic communication or any of its attachments
contain information that is not in the public domain, such information
is considered by MedImmune to be confidential and proprietary, and
expected to be used only by the individual(s) for whom it is intended.
If you have received this electronic communication in error, please
reply to the sender advising of the error in transmission and delete the
original message and any accompanying documents from your system
immediately, without copying, reviewing or otherwise using them for any
purpose. Thank you for your cooperation.





To the extent this electronic communication or any of its attachments contain
information that is not in the public domain, such information is considered by
MedImmune to be confidential and proprietary.  This communication is expected to
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have received this electronic communication in error, please reply to the sender
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or otherwise using them for any purpose.  Thank you for your cooperation.
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------------------------------

Message: 3
Date: Mon, 12 May 2008 12:56:58 -0700 (PDT)
From: Karla Arrington 
Subject: [Histonet] ICC problem
To: histonet@lists.utsouthwestern.edu
Message-ID: <251789.53346.qm@web32506.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Hello all.. 
I currently perform IHC's by hand.  Although they are beautiful, I do get 
a faint background staining inside the circle of the hydropholic pen.  It 
looks like excess DAB stain. I do not wipe around the tissues causing no
static.  Any suggestions on how to fix this? 
Karla Arrington


 
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------------------------------

Message: 4
Date: Mon, 12 May 2008 13:04:05 -0700 (PDT)
From: Karla Arrington 
Subject: [Histonet] Bone Marrow Staining
To: histonet@lists.utsouthwestern.edu
Message-ID: <92592.51349.qm@web32502.mail.mud.yahoo.com>
Content-Type: text/plain; charset=us-ascii

I currently use 10% NBF for fixing bone marrows (core and clot). Although lately
the sections appear "washed out".  I previously used B-5 Fixative.  Is there a
better
fixative for fixing bone marrows so their cells are crisp and clear? 

Karla Arrington
freckles9660@yahoo.com



 
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------------------------------

Message: 5
Date: Mon, 12 May 2008 13:17:13 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] Bone Marrow Staining
To: Karla Arrington ,
	histonet@lists.utsouthwestern.edu
Message-ID: <604837.79006.qm@web65710.mail.ac4.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

The "washed out" appearance most likely has nothing to do with fixation. Check
if you are heating the sections BEFORE they are completely drained off. That can
cause that artifact.
  The only thing you have to be aware of when using NBF to fix BM specimens is
to control the pH on the staining solutions (specially the Giemsa).
  René J.

Karla Arrington  wrote:
  I currently use 10% NBF for fixing bone marrows (core and clot). Although
lately
the sections appear "washed out". I previously used B-5 Fixative. Is there a
better
fixative for fixing bone marrows so their cells are crisp and clear? 

Karla Arrington
freckles9660@yahoo.com



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Message: 6
Date: Mon, 12 May 2008 16:33:38 -0400
From: "Victoria Baker" 
Subject: Re: [Histonet] IHC Background Staining
To: "Karla Arrington" 
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
	<4f016b690805121333m20c00719v77da5f74d5645c28@mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

What everyone else is saying is true and you will need to check your
procedure carefully.  Manual staining is a technique that is difficult
with some antibodies and especially when working with a large slide
number.

One of the things that I did was eliminate using a pap pen.  I used 2
X 2 strips of parafilm that I cut in strips of 1 X 2 and layed them on
the tissue/slide after the reagent has been applied.  To remove I
dipped the slides in buffer and the film would slide right off.

Good luck.  I did IHC manually for a long time and it is not always easy.

Vikki

On 5/9/08, Karla Arrington  wrote:
> I do Immuno's by hand and I am getting background DAB precipitate on the
inside circle of my sections.
> What is causing this and how do I get rid of it?
>
>
>
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------------------------------

Message: 7
Date: Mon, 12 May 2008 14:27:43 -0700 (PDT)
From: Teisha Robertson 
Subject: [Histonet] non specific staining
To: histonet@lists.utsouthwestern.edu
Message-ID: <605614.77385.qm@web62515.mail.re1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

how do you eliminate non specific staining in olfactory bulb sections?
       
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Message: 8
Date: Mon, 12 May 2008 16:39:43 -0500
From: "Ingles Claire" 
Subject: RE: [Histonet] RE: Latin - Thank goodness!
Cc: 
Message-ID:
	
<08A0A863637F1349BBFD83A96B27A50A120128@uwhis-xchng3.uwhis.hosp.wisc.edu>
	
Content-Type: text/plain;	charset="iso-8859-1"

I'm a youngster, but I also bought a latin dictionary just for the medical
terminology aspect. Good thing I didn't have to take it. It is interesting
stuff, but I'm terrible at learning languages.
 
Claire

________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Smith, Allen
Sent: Mon 5/12/2008 11:51 AM
To: 'Breeden, Sara'
Cc: 'histonet@lists.utsouthwestern.edu'
Subject: [Histonet] RE: Latin - Thank goodness!



Latin is also useful in law. (A sponge left in the pelvic cavity is a case of
"Res ipsa loquitur.")  Latin also helps in puzzling out articles in Spanish or
Portuguese.  Most of all Latin gives access to a great literature that has been
rather poorly translated.  No translation of the Aeneid give one any conception
of the magnificence of the original.







------------------------------

Message: 9
Date: Mon, 12 May 2008 15:52:06 -0700
From: "Thomas Jasper" 
Subject: RE: RE: [Histonet] I need more ammunition
To: "WAYNE HOLLAND" 
Cc: histonet@lists.utsouthwestern.edu
Message-ID:
	<90354A475B420441B2A0396E5008D4965E20AF@copc-sbs.COPC.local>
Content-Type: text/plain; charset="US-ASCII"

Hi Wayne,

I know I already sent you a reply on this topic (off-line).  Just had
one more thought...you might want to point out to the "powers that be"
that your organization is running a HUGE risk for the ULTIMATE in legal
nightmares.  Nothing gets the attention of "higher ups" quicker than
$$$$$$'s and lawsuits.  In the end Wayne, if you do not get anywhere
with this group, I would seriously consider seeking employment
elsewhere.  That's not always the most desirable move, but let's face
it...you are currently in a position and a market, which makes you a hot
commodity.  Secondly, you don't need to be implicated in any legal
entanglements due to poor decisions (or lack of them) by the folks in
authority where you work.  Watch out for yourself man it's tough to
speak truth to power.  I pity the poor patients as well.

Good luck,
Tom J.

Thomas Jasper HT (ASCP) BAS
Histology Supervisor
Central Oregon Regional Pathology Services
Bend, Oregon 97701
541/693-2677
tjasper@copc.net 



-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of WAYNE
HOLLAND
Sent: Saturday, May 10, 2008 5:31 PM
To: Histonet@lists.utsouthwestern.edu
Subject: FW: RE: [Histonet] I need more ammunition

I need more ammunition, please!!

>From: WAYNE HOLLAND
>Sent: Fri 5/9/2008 1:47 AM
>To: Histonet@lists.utsouthwestern.edu
>Subject: [Histonet] I need your help
>
>
>Everyone, I have started a new job. I have many things that need fixed.

>I have a gross room that are using regular cassettes metal tops and 
>they are wrapping all of the derm work in yes wet lens paper and 
>leaving them on the countertop by the hoods for periods of up to 45 
>minutes. I know this is not good for obvious reasons. I need your 
>comments asap to help make them understand, again for obvious reasons. 
>I have been doing this for 28 years and I need other field associates 
>to back me up. Most of these specimen are very small. HELP!
>
>
>
>_______________________________________________
>Histonet mailing list
>Histonet@lists.utsouthwestern.edu
>http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>NOTICE OF CONFIDENTIALITY
>This electronic message, including attachments, is for the sole use of 
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------------------------------

Message: 10
Date: Mon, 12 May 2008 16:26:06 -0700
From: "Oto, Carrie" 
Subject: [Histonet] Method validation
To: "'histonet@lists.utsouthwestern.edu'"
	
Message-ID:
	
<00EC53C1C120524B80A74AC951675233187E2C@CB-LIS-APSVR-1.ucsfmedicalcenter.org>
	
Content-Type: text/plain

Our Histology lab will be moving from one campus to another, 2 seperate
CLIA's.  Accrediting agency is Joint Commission.  What, if any, has other
labs done for method validation of histology equipment and reagents?  For
example, processor, stainer, IPOX stains, etc.  Any and all information is
appreciated especially if there is specific information for California labs.



------------------------------

Message: 11
Date: Mon, 12 May 2008 20:56:18 -0500
From: "Joe Nocito" 
Subject: Re: [Histonet] C =Con(with) S means Sans(without)-Latin
	origin
To: "Bonner, Janet" ,	"Madary, Joseph"
	,	
Message-ID: <006b01c8b49c$898769b0$0302a8c0@yourxhtr8hvc4p>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

yeah,
you go girl

JTT
----- Original Message ----- 
From: "Bonner, Janet" 
To: "Madary, Joseph" ; 

Sent: Monday, May 12, 2008 12:19 PM
Subject: RE: [Histonet] C =Con(with) S means Sans(without)-Latin origin


OK, I use w/ = with, and w/o = without.  -American

________________________________

From: histonet-bounces@lists.utsouthwestern.edu on behalf of Madary, Joseph
Sent: Mon 5/12/2008 1:08 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] C =Con(with) S means Sans(without)-Latin origin





Nick Madary, HT/HTL(ASCP)QIHC
Medimmune Histology Laboratory
One Medimmune Way
Gaithersburg, MD 20878

ph 301.398.4745/6360
fx  301.398.9745

To the extent this electronic communication or any of its attachments
contain information that is not in the public domain, such information
is considered by MedImmune to be confidential and proprietary, and
expected to be used only by the individual(s) for whom it is intended.
If you have received this electronic communication in error, please
reply to the sender advising of the error in transmission and delete the
original message and any accompanying documents from your system
immediately, without copying, reviewing or otherwise using them for any
purpose. Thank you for your cooperation.





To the extent this electronic communication or any of its attachments 
contain information that is not in the public domain, such information is 
considered by MedImmune to be confidential and proprietary.  This 
communication is expected to be read and/or used only by the individual(s) 
for whom it is intended.  If you have received this electronic communication 
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and delete the original message and any accompanying documents from your 
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------------------------------

Message: 12
Date: Tue, 13 May 2008 02:37:16 -0400
From: John Kiernan 
Subject: Re: [Histonet] 4% paraformaldehyde all over again
To: Jackie M O'Connor 
Cc: Histonet ,
	histonet-bounces@lists.utsouthwestern.edu
Message-ID: 
Content-Type: text/plain; charset="us-ascii"


   There  is no such thing as 4% paraformaldehyde. Nobody can sell or
   us   nauseam.   said,  in  3  words,   vomit".   Latin   is   concise
formaldehyde is a real solution, and    solution  is  to  heat  40 grammes
of    litre  of  water,  with  an  alkaline  catalyst  to  sp   hydrolysis.
Paraformaldehyde  exists only as a solid substan   which  is  insoluble  in
water.  All this has been in all    the textbooks and manuals for 40+
years.
   
 
Joh UWO
London, Canada
Original Message -----
From: <Jackie.O'Connor@abbott.com>< Monday, May 12, 2008 10:52
Subject: [Histo 4% paraformaldehyde all over again
To: Histonet < Histonet@lists.utsouthwestern.edu>, histonet-bounces@li sts.utsouthwestern.edu

> Will vendors who cont quantities < again, please. <
> Jackie O'Conn Labs
> _________ _______________________ 5F Histonet mail Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 13 Date: Tue, 13 May 2008 11:02:22 +0200 From: "louise renton" Subject: [Histonet] thanks To: Histonet@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset=ISO-8859-1 thanks to all who took the time to respond to my OT question. Now i can sleep nights.......... -- Louise Renton Bone Research Unit University of the Witwatersrand Johannesburg South Africa "There are nights when the wolves are silent and only the moon howls". George Carlin No trees were killed in the sending of this message. However, many electrons were terribly inconvenienced. ------------------------------ Message: 14 Date: Tue, 13 May 2008 09:07:41 -0400 From: "Smith, Allen" Subject: RE: [Histonet] 4% paraformaldehyde all over again To: 'John Kiernan' Cc: "'histonet@lists.utsouthwestern.edu'" Message-ID: Content-Type: text/plain; charset="us-ascii" It appears that Dr. Kiernan is another victim of Microsoft office 2007. Could a users' coalition large enough to boycott it effectively be formed? -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of John Kiernan Sent: Tuesday, May 13, 2008 2:37 AM To: Jackie M O'Connor Cc: Histonet; histonet-bounces@lists.utsouthwestern.edu Subject: Re: [Histonet] 4% paraformaldehyde all over again There is no such thing as 4% paraformaldehyde. Nobody can sell or us=uch a non-substance!
See Histonet archives passim ad nauseam.=6nbsp; (That's how the ancient Romans said, in 3 words,=2on and on, until you want to vomit". Latin is concise=
 
4% formaldehyde is a real solution, and =e of the ways of making the solution is to heat 40 grammes of =raformaldehyde in a litre of water, with an alkaline catalyst to sp?d up the hydrolysis. Paraformaldehyde exists only as a solid substan?, which is insoluble in water.  All this has been in all the textbooks and manuals for 40+ years.
 
Joh=iernan
Anatomy,  UWO
London, Canada
=D = = = =
----- Original Message -----
From: =ckie M O'Connor <Jackie.O'Connor@abbott.com><=>Date: Monday, May 12, 2008 10:52
Subject: [Histo=t] 4% paraformaldehyde all over again
To: Histonet < Histonet@lists.utsouthwestern.edu>, histonet-bounces@li sts.utsouthwestern.edu

> Will vendors who cont?ted me off line regarding supplying vast
> quantities <=>> of 4% paraformaldehyde contact me again, please. <=>> Thanks.
>
> Jackie O'Conn=
> Abbott Labs
> _________ _______________________ 5F=F_____________
> Histonet mail=g list
> Histonet@lists.utsouthwestern.edu
=6gt; http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 15 Date: Tue, 13 May 2008 10:49:40 -0400 From: "Amy Porter" Subject: [Histonet] Catalase Staining for Liver Aspirates Help Please To: Cc: miyakaw2@msu.edu Message-ID: <001201c8b508$92a98c50$8e7a0923@histolab> Content-Type: text/plain; charset="iso-8859-1" Hello to all - I am looking for an enzyme staining method to demonstrate peroxisomes (catalase specifically) in Canine Liver aspirate smears. If anyone out there has anything they would be will to share it would be so appreciated. I have spent a great deal of time searching on the web and have not yet found a methodology. Thanks in advance for any assistance. Amy Amy S. Porter, HT (ASCP) QIHC Investigative HistoPathology Laboratory - Supervisor 2201 Biomedical Physical Sciences Bldg. Rm #2133 East Lansing, MI 48824-3320 Phone: (517) 884-5026 Fax: (517) 432-1368 Email: portera@msu.edu Web: www.humanpathology.msu.edu ------------------------------ Message: 16 Date: Tue, 13 May 2008 11:23:17 -0400 From: "Angela Bitting" Subject: [Histonet] Amended reports- Cerner CoPath users To: Message-ID: <48297A25.2B7F.00C9.0@geisinger.edu> Content-Type: text/plain; charset="us-ascii" I've got a question for users of Cerner Copath. Do you have CoPath set up so that when a report is amended the original diagnosis remains visible along with the amended diagnosis? Also, is there some type of flag at the top of the report to alert a physician that there have been changes to the report? Thanks for your help on this one. Angie Angela Bitting, HT(ASCP) Technical Specialist, Histology Geisinger Medical Center 100 N Academy Ave. MC 23-00 Danville, PA 17822 phone 570-214-9634 fax 570-271-5916 No trees were hurt in the sending of this email However many electrons were severly inconvienienced! IMPORTANT WARNING: The information in this message (and the documents attached to it, if any) is confidential and may be legally privileged. It is intended solely for the addressee. Access to this message by anyone else is unauthorized. If you are not the intended recipient, any disclosure, copying, distribution or any action taken, or omitted to be taken, in reliance on it is prohibited and may be unlawful. If you have received this message in error, please delete all electronic copies of this message (and the documents attached to it, if any), destroy any hard copies you may have created and notify me immediately by replying to this email. Thank you. -------------- next part -------------- BEGIN:VCARD VERSION:2.1 X-GWTYPE:USER FN:Bitting, Angela TEL;WORK:570-271-6844 ORG:;Histology EMAIL;WORK;PREF;NGW:AKBITTING@geisinger.edu N:Bitting;Angela END:VCARD ------------------------------ Message: 17 Date: Tue, 13 May 2008 11:28:24 -0500 From: Nancy Schmitt Subject: [Histonet] Standardized Microtomes To: "'histonet@lists.utsouthwestern.edu'" Message-ID: <9FC023A4AB52BB4D87DC6456081A822C087C8D@mercury.pa-ucl.com> Content-Type: text/plain; charset="iso-8859-1" Good Morning to all Does anybody standardize microtomes so they all cut at same angle? Is this impossible? We only cut control blocks on one microtome because they are each at a little different angle. Would be nice to be able to do at all........... Thanks for any feedback Nancy Schmitt Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. ------------------------------ Message: 18 Date: Tue, 13 May 2008 11:30:13 -0500 From: "Michele Wich" Subject: [Histonet] Apipophilin and MCM To: Message-ID: <62A8156F8071C8439080D626DF8C33A602E3F5@wave-mail.7thwave.local> Content-Type: text/plain; charset="US-ASCII" Does anyone know if Apipophilin and MCM (mini chromosome maintenance protein) are commercially available and, if so, who sells them? Thanks for any info! This communication is intended solely for the use of the addressee and may contain information that is legally privileged, confidential or exempt from disclosure. If you are not the intended recipient, please note that any dissemination, distribution, or copying of this communication is strictly prohibited. Anyone who receives this message in error should notify the sender immediately and delete it from his or her computer ------------------------------ Message: 19 Date: Tue, 13 May 2008 11:35:58 -0500 From: Atoska Gentry Subject: [Histonet] rubber stoppers & tubing To: Histonet Message-ID: <4829C36E.8000504@vetmed.auburn.edu> Content-Type: text/plain; charset=ISO-8859-1; format=flowed hello, if any of you have a source for two-hole black rubber stoppers measuring : 21mm top x 13mm bottom x 7mm in length ( with the inscription 304w SET on top, and with one hole measuring 2-3mm and the other 3-4 mm); and or 15mm bottom x 8mm in length; also, rigid plastic tubing 2-3 mm in diameter, 196mm in length will you please contact me ASAP? Thank you kindly, Atoska -- Atoska S. Gentry, B.S., HT(ASCP) Research Assistant IV Scott-Ritchey RSCH Center College of Vet. Med Auburn, AL 36849 PH (334) 844-5579 FAX (334) 844-5850 email: gentras@vetmed.auburn.edu ------------------------------ Message: 20 Date: Tue, 13 May 2008 10:38:18 -0600 From: "Liz Chlipala" Subject: RE: [Histonet] Standardized Microtomes To: "Nancy Schmitt" , Message-ID: Content-Type: text/plain; charset="US-ASCII" Newcomer supply has a device that will standardize microtomes. Liz Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC Manager Premier Laboratory, LLC P.O. Box 18592 Boulder, CO 80308 phone (303) 682-3949 fax (303) 682-9060 liz@premierlab.com www.premierlab.com Ship to Address: Premier Laboratory, LLC 1567 Skyway Drive Unit E Longmont, CO 80504 -----Original Message----- From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Nancy Schmitt Sent: Tuesday, May 13, 2008 10:28 AM To: 'histonet@lists.utsouthwestern.edu' Subject: [Histonet] Standardized Microtomes Good Morning to all Does anybody standardize microtomes so they all cut at same angle? Is this impossible? We only cut control blocks on one microtome because they are each at a little different angle. Would be nice to be able to do at all........... Thanks for any feedback Nancy Schmitt Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 21 Date: Tue, 13 May 2008 11:41:10 -0500 From: Jackie M O'Connor Subject: Re: [Histonet] Standardized Microtomes To: Nancy Schmitt Cc: "'histonet@lists.utsouthwestern.edu'" , histonet-bounces@lists.utsouthwestern.edu Message-ID: Content-Type: text/plain; charset="US-ASCII" Get a block aligner. We have aligned all of our microtomes so that any technicican can do a recut no matter what machine it was originally cut on. Nancy Schmitt Sent by: histonet-bounces@lists.utsouthwestern.edu 05/13/2008 11:28 AM To "'histonet@lists.utsouthwestern.edu'" cc Subject [Histonet] Standardized Microtomes Good Morning to all Does anybody standardize microtomes so they all cut at same angle? Is this impossible? We only cut control blocks on one microtome because they are each at a little different angle. Would be nice to be able to do at all........... Thanks for any feedback Nancy Schmitt Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ------------------------------ Message: 22 Date: Tue, 13 May 2008 11:54:13 -0500 From: amy rizzo Subject: [Histonet] Paraffin thickness standard or measurement? To: Message-ID: <000001c8b519$f8d86440$c91e3086@marqnet.mu.edu> Content-Type: text/plain; charset="us-ascii" Is there anyway to measure the thickness of your paraffin section as it comes off a microtome? I have noticed mine isn't as consistent as I would like. Do they sell something anywhere that does this? Thanks, Amy Rizzo ------------------------------ Message: 23 Date: Tue, 13 May 2008 12:56:55 -0400 From: "Joyce Cline" Subject: RE: [Histonet] Standardized Microtomes To: Message-ID: <001201c8b51a$5996a080$1d2a14ac@wchsys.org> Content-Type: text/plain; charset="us-ascii" Each week we align all our microtomes. A company called Advance Innovations has created an aligner that is available through several companies. Aligning our microtomes prevents losing tissue from small biopsies when a recut is called for. ++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++++ Good Morning to all Does anybody standardize microtomes so they all cut at same angle? Is this impossible? We only cut control blocks on one microtome because they are each at a little different angle. Would be nice to be able to do at all........... Thanks for any feedback Nancy Schmitt Histology Coordinator Dubuque, IA NOTICE: This email may contain legally privileged information. The information is for the use of only the intended recipient(s) even if addressed incorrectly. If you are not the intended recipient, please notify the sender that you have received it in error and then delete it along with any attachments. Thank you. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet ***** CONFIDENTIALITY NOTICE ***** This message contains confidential information and is intended only for the individual named. If you are not the named addressee you should not disseminate, distribute or copy this e-mail. Please notify the sender immediately by e-mail if you have received this e-mail by mistake and delete this e-mail from your system. ------------------------------ _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet End of Histonet Digest, Vol 54, Issue 17 **************************************** The information transmitted in this electronic communication is intended only for the person or entity to whom it is addressed and may contain confidential and/or privileged material. Any review, retransmission, dissemination or other use of or taking of any action in reliance upon this information by persons or entities other than the intended recipient is prohibited. If you received this information in error, please contact the Compliance HelpLine at 800-856-1983 and properly dispose of this information. _______________________________________________ Histonet mailing list Histonet@lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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