Re: [Histonet] processing fatty tissue

From:Rene J Buesa



Robert:
  Under separate cover I am sending you details of my procedure. I am Sure Maxim will do the same.
  RenÚ J.

Robert Chiovetti  wrote:
  Maxim, Rene (and Other Histonetters),

That's interesting re: using either a mix of ethanol+isopropanol+mineral oil (Rene) or isopropanol+mineral oil (Maxim) for breast tissue. Could you share your recipes with us? 

I have a customer (derm path) who could probably benefit from this for larger and thicker skin specimens which sometimes have a lot of subcutaneous fat associated with them.

Thanks in advance, if you could share the recipes!

Cheers,

Bob

Robert (Bob) Chiovetti, Ph.D.
Southwest Precision Instruments
The Desert Southwest's Microscopy Resource
Tucson, Arizona USA
Tel./Fax 520-546-4986
www.swpinet.com
Member, Arizona Small Business Association
(www.asba.com)

----- Original Message ----
From: Maxim Peshkov 
To: ROrr@enh.org
Cc: histonet@lists.utsouthwestern.edu; Gervaip@aol.com
Sent: Thursday, May 31, 2007 12:05:21 PM
Subject: Re: [Histonet] processing fatty tissue

I completely agree with Rene.
When we processed breasts (and other tissues) with
isopropanol+mineral oil that we ceased to feel
the difficulties with our samples.
We have manually processing.
More thanks to Dr. McCormick for cassettes!
Sincerely,
Maxim Peshkov
Russia
Taganrog


"Rene J Buesa" wrote:
Date: Tue, 29 May 2007 06:22:59 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] processing fatty tissue
To: "Orr, Rebecca" ,
histonet@lists.utsouthwestern.edu
Cc: Gervaip@aol.com

1- thin slices
2- well fixed after the slices are prepared (at
least 8 h)
3- use fresh alcohols for dehydration
4- extend somewaht (20%) the clearing steps
time (time x 1.2), and
5- if possible use a 60˘C paraffin, OR
process with ethanol followed by a mixture of
ethanol+propanol+mineral oil, and breats will cut
"like butter".
Ren┘ J.








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