Re: [Histonet] processing fatty tissue

From:Rene J Buesa

  Under separate cover I am sending you details of my procedure. I am Sure Maxim will do the same.
  RenÚ J.

Robert Chiovetti  wrote:
  Maxim, Rene (and Other Histonetters),

That's interesting re: using either a mix of ethanol+isopropanol+mineral oil (Rene) or isopropanol+mineral oil (Maxim) for breast tissue. Could you share your recipes with us? 

I have a customer (derm path) who could probably benefit from this for larger and thicker skin specimens which sometimes have a lot of subcutaneous fat associated with them.

Thanks in advance, if you could share the recipes!



Robert (Bob) Chiovetti, Ph.D.
Southwest Precision Instruments
The Desert Southwest's Microscopy Resource
Tucson, Arizona USA
Tel./Fax 520-546-4986
Member, Arizona Small Business Association

----- Original Message ----
From: Maxim Peshkov 
Sent: Thursday, May 31, 2007 12:05:21 PM
Subject: Re: [Histonet] processing fatty tissue

I completely agree with Rene.
When we processed breasts (and other tissues) with
isopropanol+mineral oil that we ceased to feel
the difficulties with our samples.
We have manually processing.
More thanks to Dr. McCormick for cassettes!
Maxim Peshkov

"Rene J Buesa" wrote:
Date: Tue, 29 May 2007 06:22:59 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] processing fatty tissue
To: "Orr, Rebecca" ,

1- thin slices
2- well fixed after the slices are prepared (at
least 8 h)
3- use fresh alcohols for dehydration
4- extend somewaht (20%) the clearing steps
time (time x 1.2), and
5- if possible use a 60˘C paraffin, OR
process with ethanol followed by a mixture of
ethanol+propanol+mineral oil, and breats will cut
"like butter".
Ren┘ J.

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