Louise,

We start with a target concentration of 10 ug/ml, and I have seen 20ug/ml 
per DAKO/Boenisch Manual recommendation too, then do serial dilutions.

  At 01:35 AM 5/29/2007, you wrote:
>Hi
>this is further to a query I submitted last week. If starting to optimise a
>new antibody that has no dilution data supplied, what is a reasonable
>concentration  to start off with.?  Antibody is supplied at 0.2mg/ml and is
>polyclonal.

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610




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