Jacqui:
  You are referring to the Michaelis veronal buffer for mast cells staining (Leder procedure).
  Unfortunately (as far as I know) this procedure works only with the veronal buffer (pH 6.3) and it is a component difficult to find (since it is a controlled substance).
  If you work in a hospital perhaps the pharmacy could have sodium barbital which is the difficult component to find.
  René J.

Jacqui Detmar  wrote:
  Hi all. I would like to do some acid phosphatase staining, but the
protocol I have includes a buffer that calls for sodium barbitone. I
have searched a number of different sites for a possible substitute
(including Histonet archives) and I can't seem to find one. Does anyone
know if I can substitute another buffer for the veronal-acetate buffer?




Thanks,



Jacqui Detmar, Ph.D. candidate

Samuel Lunenfeld Research Insitute, room 876

Mount Sinai Hospital

600 University Avenue

Toronto, ON, Canada

M5G 1X5





_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




       
---------------------------------
Get the free Yahoo! toolbar and rest assured with the added security of spyware protection. 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet