I have an old Tissue Tek II as the "back-up" cryostat. I monitor its temperature everyday and found that the temperature I have it set at and the actual temp varies considerably. I had our bio-med guy look at it and he said that there really isn't any thing he could do about it. So I watch it closely. I noticed that as frost starts to build up, the tempreature goes up. I know it's time to defrost it when the temp gets out of range. Once newly defrosted, it goes back to the colder end of the range. I have a seperate thermometer inside the chamber to get an acurate reading for my QA log. I do have a manual (copyright 1966!!) and it doen't say anything about setting the temperature.
Hope this helps,
------- Original message --------------
From: "Heckford, Karen - SMMC-SF"
> Good Morning Everyone, We have a old Tissue Tek 11 4553 cryostat. We
> noticed after defrosting it that the temperature went way cold. We turned
> the dial which moves the black hand and set it for -25 and noticed that the
> blue hand is way past -30. We cannot find the users guide to save our
> lives. Probably lost a long time ago. Anyways can anyone tell me how to
> reset this thing.
> Karen Heckford HT (ASCP) CE
> Lead Histology Technician
> Histology/Pathology Department
> St. Mary's Medical Center
> 450 Stanyan St.
> San Francisco, Ca. 94117
> 415-668-1000 ext. 6167
> Fax: 415-750-8123
> email: email@example.com