Re: [Histonet] RE: NK cell on mouse tissue

From:Gayle Callis

This NK1.1 (P136) mouse on mouse is not recommended for use with 
immunohistochemistry according to BD Bioscience technical data sheet.   I 
am curious who has published success though, same as you and mentioned by 
Sonya)?  Maybe she can provide the references on this.

PLP stands for periodate lysine paraformaldehyde (McKean et al).

  At 12:01 PM 5/22/2007, you wrote:
>Hi Sonya,
>Funny that you ask, I have just run across this problem myself. I also
>don't have much luck using (various) NK1.1 markers on frozens. (I had
>one that worked fairly well, and tried again last week after some time
>that no longer works, ordered fresh, and still nothing.)
>I have been using CD56 from US Biological for a couple years now with
>consistent results.
>I am not familiar with PLP fixation.. what does it stand for and what is
>the reference for using it for NK's in particular?
>Are you getting any staining on your positive controls?

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610

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