Have you checked the pH of your solutions? What about the temperature of the water baths? Are your times accurate in the pretreatments and hybridization and posthyb? What about collection of the specimen, are you certain they are properly trained and the specimen are arriving cold and in the proper fixative?
Roxanne Soto HT(ASCP)QIHC
From: Lester Raff
Sent: Tue, 22 May 2007 11:04 am
Subject: [Histonet] Please ask your colleagues doing FISH
There is no current list serve for FISH (one is being developed) so I am
sking histonetters for help. Please pass this on to anyone in your
abs doing FISH testing.
We are performing UroVysion FISH testing for bladder cancer. Lately we
re seeing a strange orange red glow on our slides under the fluorescent
cope. It is most noticeable on triple filter and gold filter. Abbott
as offered some suggestions, but we are still having problems. Any
Lester J. Raff, MD
roPartners, LLC Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, IL 60154
istonet mailing list
AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com.
Histonet mailing list