Have you checked the pH of your solutions?  What about the temperature of the water baths?  Are your times accurate in the pretreatments and hybridization and posthyb?  What about collection of the specimen, are you certain they are properly trained and the specimen are arriving cold and in the proper fixative?

Roxanne Soto HT(ASCP)QIHC


-----Original Message-----
From: Lester Raff 
To: histonet@lists.utsouthwestern.edu
Sent: Tue, 22 May 2007 11:04 am
Subject: [Histonet] Please ask your colleagues doing FISH



Hello Histonetters:
 
There is no current list serve for FISH (one is being developed) so I am
sking histonetters for help.  Please pass this on to anyone in your
abs doing FISH testing.
 
We are performing UroVysion FISH testing for bladder cancer.  Lately we
re seeing a strange orange red glow on our slides under the fluorescent
cope.  It is most noticeable on triple filter and gold filter.  Abbott
as offered some suggestions, but we are still having problems.  Any
uggestions???
 
Lester J. Raff, MD
edical Director
roPartners, LLC Laboratory
2225 Enterprise Dr. Suite 2511
Westchester, IL 60154

h:  708-486-0076
ax: 708-486-0080
 
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