Re: [Histonet] IHC cytology specimens

From:Rene J Buesa

If you use formalin will have to do HIER and the smears will not "survive" it.
  Use alcohol as fixative¡úair dry¡úPBS¡úIHC
  Ren¨¦ J.

"Deathridge, Mary Ann"  wrote:
  Does anyone in histoland have a protocol for the prep of cytology specimens (cytospins, smears) for IHC?
Formalin fix? how long? air dried? 95% alc fix? esp. since the new Her2nu guidelines
Thanks in advance

MaryAnn Deathridge, HT (ASCP)
Supervisor, Histopathology
Vanderbilt Un. Med. Ctr
Nashville, TN
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