Re: [Histonet] Help with MDM2 Protein Immunostaining

From:"Dana Settembre"

Hello Young Kwun,

I use MDM2 from Dako cat.#  M7146
It is IVD.
My pathologist seems satisfied with this.
I use Dako's Target Retreival Solution in a steamer for 40 minutes.
I use it at 1:40.
Good Luck,

Dana Settembre, HT ASCP
Immunohistochemistry Lab
UMDNJ - University Hospital
Newark, NJ    USA

>>> Young Kwun  05/30/07 7:53 PM >>>
Dear Colleagues,


Does anyone in Histoland use MDM2 Protein staining for some soft
sarcomas successfully?

I tried Lab Vision's polyclonal antibody (1/100 dil) and Novocastra's
(clone 1B10, 1/150 dil) with Decloaker HIER and BondMax Autostainer.

However, my pathologist is not happy with the staining results, mainly
because of some non-specific stains. 

Any help would be appreciated. Thank you.




Young Kwun

Senior Hospital Scientist

Dept of Anatomical Pathology

Concord Hospital

Concord NSW 2139


Phone:61-2-9767 6075

Fax:61-2-9767 8427 




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