Re: [Histonet] Fwd: antibody concentration..oops

From:Rene J Buesa



Just as a guide, check other policlonals you use that also have 0.2 mg/mL concentration of IgG and start with the sames concentrations you use on them to start your "checker-board" dilutions tests.
  René J.

louise renton  wrote:
  Hi
this is further to a query I submitted last week. If starting to optimise a
new antibody that has no dilution data supplied, what is a reasonable
concentration to start off with.? Antibody is supplied at 0.2mg/ml and is
polyclonal. This is for immunohistochem on FFPE slides

many thanks
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.


-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.
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