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You can replace the barbitone buffer with an acetate buffer:
About 20ml 0.2M Acetic acid plus 30ml 0.2M Sodium Acetate and pH to 4.8
(the pH we use for our acid phosphatases).
Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Jacqui
Detmar
Sent: Monday, 28 May 2007 7:13 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] Veronal-acetate buffer
Hi all. I would like to do some acid phosphatase staining, but the
protocol I have includes a buffer that calls for sodium barbitone. I
have searched a number of different sites for a possible substitute
(including Histonet archives) and I can't seem to find one. Does anyone
know if I can substitute another buffer for the veronal-acetate buffer?
Thanks,
Jacqui Detmar, Ph.D. candidate
Samuel Lunenfeld Research Insitute, room 876
Mount Sinai Hospital
600 University Avenue
Toronto, ON, Canada
M5G 1X5
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