RE: [Histonet] Veronal-acetate buffer

From:"Tony Henwood"

This email and any files transmitted with it are confidential and intended solely for the use of the individual or entity to whom they are addressed. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message and any attachments are those of the individual sender, and are not necessarily the views of The Children's Hospital at Westmead

This note also confirms that this email message has been
virus scanned and although no computer viruses were detected, The Childrens Hospital at Westmead accepts no liability for any consequential damage resulting from email containing computer viruses.

You can replace the barbitone buffer with an acetate buffer:

About 20ml 0.2M Acetic acid plus 30ml 0.2M Sodium Acetate and pH to 4.8
(the pH we use for our acid phosphatases).


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
[] On Behalf Of Jacqui
Sent: Monday, 28 May 2007 7:13 AM
Subject: [Histonet] Veronal-acetate buffer

Hi all.  I would like to do some acid phosphatase staining, but the
protocol I have includes a buffer that calls for sodium barbitone.  I
have searched a number of different sites for a possible substitute
(including Histonet archives) and I can't seem to find one.  Does anyone
know if I can substitute another buffer for the veronal-acetate buffer?




Jacqui Detmar, Ph.D. candidate

Samuel Lunenfeld Research Insitute, room 876

Mount Sinai Hospital

600 University Avenue

Toronto, ON, Canada

M5G 1X5



Histonet mailing list

Histonet mailing list

<< Previous Message | Next Message >>