Dear Ian,
On the subject of rapid freezing, I would like to make you aware that we
manufacture the Bright Clini-RF Ultra low rapid freezer which is being
used as a replacement for CO2 &
liquid nitrogen. At present we have one type that's rapid freezing tank
operates at -80 degs C and will shortly have another model for -100 degs
C. There are also options available
for controlling the temperature too.
Best Regards
Alan Bright
Bright Instrument Co.Ltd.
St Margaret's Way
Huntingdon
Cambridgeshire
PE29 6EU
England
Tel No:+44 (0)1480 454528
Fax No:+44 (0)1480 456031
Email: abright@brightinstruments.com
Web Site: www.brightinstruments.com
Skype: dazzle0
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Ian
Montgomery
Sent: 25 May 2007 11:54
To: Histonet
Subject: [Histonet] Neurogenesis
I'm just about to start my literature search for information on a
new
neurogenesis project. In the first instance I'll only be looking at
markers
for new neurones so any hints and tips pointing me in the right
direction
would be welcome.
Recently a topic has covered freezing tissue in iso-pentane cooled
with
solid CO2. This is also of interest for another project where I'll be
studying skeletal muscle from salmon. My routine technique is
iso-pentane
cooled using liquid nitrogen and for an earlier pilot study this was the
technique used. But, these salmon specimens are taken from pens in
Scottish
sea lochs so technically it's a wee bit tricky for the scientists
freezing
the specimens. Remember, this is Scotland where wind, rain and heaving
seas
are normal so using liquid nitrogen presents health and safety issues. A
question for those who have frozen muscle using CO2 cooled iso-pentane?
Can
it be done successfully and repeatedly without ice-crystal damage?
Obviously
I'll get the people at the fish farm to try it before the experiments
start
but I don't want them to waste their time as in the commercial setting,
time
is money.
I should also point out that Scottish salmon is like the whisky, the
gift of the gods.
Ian.
Dr. Ian Montgomery,
Histotechnology,
IBLS Support Services,
Thomson Building,
University of Glasgow,
Tel:01413398855
Extn: 8511.
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