RE: [Histonet] Chlorine gas

From:"Edwards, R.E."

Also  known  as  Gooding  and  Stewarts(5-20% formic acid/ 5-20%
formalin), depending  if  you  are  in  a rush  or  not!, works  a

-----Original Message-----
[] On Behalf Of Smith,
Sent: 23 May 2007 14:36
To: Anne Saulsbery
Subject: RE: [Histonet] Chlorine gas

It depends on what you use for decalcification.  If you use Hollande's
fluid, formic acid, or EDTA, there is no chloride to be oxidized to
chlorine. My preference for decalcification is Schmorl's fluid
(formalin-formic acid). In theory, formalin can react with hydrochloric
acid to produce the dangerous volatile liquid bis-chloromethyl ether,
but the yield from dilute solutions at atmospheric pressure is trivial.
Nevertheless, if I mixed formalin and HCl I would do it in a fume hood.
Mixing concentrated ammonium hydroxide and sodium hypochlorite
bleach") produces a fairly good yield of chlorine: don't try it!

Allen A. Smith, Ph.D.
Professor of Anatomy
Barry University School of Graduate Medical Sciences
    Podiatric Medicine and Surgery
Miami Shores, Florida  33161

-----Original Message-----
[] On Behalf Of Anne
Sent: Tuesday, May 22, 2007 2:10 PM
Subject: [Histonet] Chlorine gas

Has any one heard that mixing decal solution and formalin would cause
the formation of chlorine gas?
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