RE: [Histonet] Cell Block preparation

From:"Tony Henwood"

I have used both agar amd plasma clot preparations for IPX.

If the sample has been fixed in solution prior to cell block preparation
then I have often had to resort to agar cell block preparations. Also if
the sample has a high concentration of proteases eg bile fluid, then
often the plasma clot won't form.

IPXs can often be problematical in agar cell blocks, probably due to the
deleterious effect of the hot agar. Plasma Cell Blocks are definitely
more consistent


Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC)
Laboratory Manager & Senior Scientist
The Children's Hospital at Westmead,
Locked Bag 4001, Westmead, 2145, AUSTRALIA.
Tel: 612 9845 3306
Fax: 612 9845 3318

-----Original Message-----
[] On Behalf Of Houston,
Sent: Wednesday, 30 May 2007 6:15 AM
Subject: [Histonet] Cell Block preparation

Would anyone care to comment on the pros and cons of preparing cell
blocks using the Plamsa/thrombin technique and the Agar method which is
more prominent in the European field?


I am particularly interested in the prevalence of background staining in
ICC. Does the use of plasma interfere with interpretation of the
staining results? I know there have been reports of extraneous tissue
being found in cell blocks coming from a commercially prepared clotting





Ronnie Houston, MS, HT(ASCP)QIHC

Anatomic Pathology Manager

Columbus Children's Hospital

700 Children's Drive

Columbus, OH 43205



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