I actually caught a Cardiology Fellow trying to embed mouse heart tissue
from formalin into paraffin. He wanted me to tell him what to do with
the formalin when he was finished. He said he was trying to save the
department research money by skipping the processing step. This was in a
major Boston institution labeled as within the "top 3" in the country!
Takes all kinds.........
TGIF and start of my VACATION! Yipee!
-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Thomas
Pier
Sent: Friday, May 18, 2007 12:42 PM
To: Jackie.O'Connor@abbott.com; histonet@lists.utsouthwestern.edu;
histonet-bounces@lists.utsouthwestern.edu
Subject: Re: [Histonet] CSI Las Vegas
Yeah, my wife had to tell me to quite down when I started shouting about
how he was doing it all wrong. The tissue was never fixed or processed.
It probably would've been totally fried from the "deparaffinization
method" that was used. He turned the wheel on the microtome the wrong
way. The slide was also magically stained. The biggest problem though,
is that a pathologist actually did his own histology. When was the last
time that anybody saw that happen?
Happy Friday,
Tom
>>> "Jackie M O'Connor" 05/18/07 10:38 AM
>>>
Anyone catch the histology scene in CSI last night? The medical
examiner
took a piece of skin from a victim, put it in a mold, poured wax on it,
put it in a microtome, turned the wheel backwards to get a section on a
slide, melted the wax off with a Bunsen burner, then looked at the
section
under the microscope - -from that he determined that the victim was
electrocuted. I love TV science.
Happy Friday!
Jackie O'
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