[Histonet] vacuoles in H&E stain

From:"Elgert, Phil"

Hi All,


Here's a stumper. We deparaffinize for 10 minutes. We use Richard Allen
hematolylin and eosin. We have 5 alcohols before going into our last
Xylenes for clearing. So no water at the end. We still have a lot of
nuclear vacuoles on the finished slides. I suspected processing but when
I sent blocks to a lab across town they turned out fine. Has anybody
seen this before? 


p.s. Have a great memorial day weekend.


Phil Elgert

Histology Supervisor

Kadlec Medical Center

888 Swift Blvd.

Richland, Wa 99352





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