I've used mouse anti CD57 ( clone NK1) on frozen, ( prefixed or fresh, then 
fixed)  and pwax sections of mouse and found that Ab reagent to be very 
good.
Doesn't help you with yours, I'm afraid.
Maybe it will work on pwax sections, after HIER?
A couple of NK-1 pics here http://www.immunoportal.com/ using above Ab on 
mouse pwax sections.
Re PLP: must admit, I have never yet used an Ab that has  needed it, rather 
than std 4% Formalin.
Then again, Ks of Abs I've never used.
Best wishes
carl 


_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet