[Histonet] RE: IHC cytology specimens

From:"Joanne Mauger"


I agree with Chris. NBF actually seems to help tissue stay on slide
better. I will post fix a smear or cytospin in NBF for 15 mins. that has
been previously fixed in alcohol. I do HIER as I would on FFPE.

I have found that some antibodies will not show expression unless this
post NBF fixation and retrieval has been done-eg. INI-1. I have also
found retrieval necessary on thin prep cytospins that had been only
fixed in sacamano,and 95% etoh.

Trial and error is the key.


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