Thanks to all that responded to this question.  It's interesting to see
a variety of reasons why acetone is used at this temp.  I was once told
by a researcher that "it's possibly due to how acetone was stored many
moons ago and the practice just carried thru since."

Roger  

 

 

Roger Charles

Microbiologist

Pennsylvania Veterinary Laboratory

2305 N Cameron St

Harrisburg, PA 17110

717-787-8808

-----Original Message-----
From: Charles, Roger 
Sent: Tuesday, May 22, 2007 2:29 PM
To: Histonet (histonet@lists.utsouthwestern.edu)
Subject: -20C acetone fixative

 

Does any know or remember why acetone, when used as a fixative, is
always used "ice cold" or in our case at -20C? 

 

 

Roger Charles

Microbiologist

Pennsylvania Veterinary Laboratory

2305 N Cameron St

Harrisburg, PA 17110

717-787-8808

 

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