[Histonet] Help with MDM2 Protein Immunostaining

From:"Young Kwun"

Dear Colleagues,


Does anyone in Histoland use MDM2 Protein staining for some soft tissue
sarcomas successfully?

I tried Lab Vision's polyclonal antibody (1/100 dil) and Novocastra's MDM2
(clone 1B10, 1/150 dil) with Decloaker HIER and BondMax Autostainer.

However, my pathologist is not happy with the staining results, mainly
because of some non-specific stains. 

Any help would be appreciated. Thank you.




Young Kwun

Senior Hospital Scientist

Dept of Anatomical Pathology

Concord Hospital

Concord NSW 2139


Phone:61-2-9767 6075

Fax:61-2-9767 8427





Histonet mailing list

<< Previous Message | Next Message >>