Re: [Histonet] reprocessing

From:Gayle Callis

Go to Histonet Archives and look for Mickie Johnson's way of reprocessing 
tissues.  I think he also published this in Histo Logic, Sakura Finetek 
website - it was a delightfully simple thing to do and you did not have to 
remove the paraffin to do it.  Hopefully, he is looking in and will help 
with this again.

At 09:35 AM 5/4/2007, you wrote:
>I am reprocessing some tissues that did not process adequately and was 
>curious as to if after they are followed backwards through xylene, 100, 
>95, 80 and 70, could the tissues go back into formalin with no adverse 
>affects? Would it be better to simply leave them in 70% for the weekend. 
>The one thing I don't want to do on a Friday afternoon is to sit around 
>and stay late to process tissues.
>Thanks for your help.
>Derek Papalegis
>T-NEMC Animal Histology Core
>Division of Laboratory Animal Medicine
>Tufts University 136 Harrison Avenue
>Boston, MA 02111
>phone: 617 636-2971
>fax: 617 636-8354
>Histonet mailing list

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610

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