Pamela Marcum wrote: The blocks should be stored in 70% alcohol as absolute will remove
all water even the bound molecules of water you actually need. Also
going into a processor for routine processing may be fixative
(aqueous) through lower grades of alcohol that replace the water just
removed by your storage alcohol and then remove it again. All of
these factors could contribute to the cracking as the tissue is
completely over exposed to alcohol.
It would help to have the actual protocol used for processing.
Pam Marcum (address at the bottom)
At 04:40 PM 5/9/2007, you wrote:
>I am having a bit of trouble obtaining good quality slides of bat
>brains. The brains are fixed in Carnoy's, stored in absolute
>alcohol, processed routinely, infiltrated with paraffin, sectioned
>at 8 microns and stained using cresyl fast violet. The aim is to
>record the cytoarchitecture. The resulting slides show poor tissue
>integrity with extensive cracking. Can anyone suggest how this can
>be avoided? I was thinking maybe a double-embedding method for
>whole brains (10-15 mm) and thicker sections (25-30 microns)?
>
> Thanking you in advance
>
> I-sanna Gibbons, DVM
> Veterinary Anatomy
> School of Veterinary Medicine
> The University of the West Indies
> Trinidad and Tobago, W.I.
>
>
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Best Regards,
Pamela A Marcum
Manager, Histology Special Procedures
University of Pennsylvania
School of Veterinary Medicine
R.S. Reynolds Jr. CORL
New Bolton Center
382 West Street Road
Kennett Square, PA 19348
Phone - 610-925-6278
Fax - 610-925-8120
E-mail - pmarcum@vet.upenn.edu
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