God forbid that you & your pathologist see the same color
differently...I go through this with my boss every once in a while. An
H&E slide will look just fine to me, but to him it's too pink, whereupon
he tells me to go clean my glasses, change out the H&E and restain the
slide. :oP Fortunately, our lab is a university research lab, so we
don't have to undergo CAP inspection.
Dept of Pharmacology & Toxicology
Philip Oshel wrote:
> Sure, why not? Require all histotechs to pass a "color-blindness"
> (note the quotes) eye exam. Given that women generally have better
> color discrimination than men in the red-green end of the spectrum,
> female histotechs should be more valuble and able to demand higher pay.
> I can just see medical supply houses all carrying Pantone color charts
>> I have followed this discussion with interest and a bit of humor on
>> the rationale for this chore. It escapes me a bit - but I did like
>> the idea of an atomic clock but then saw the reply about battery
>> failure. Also, Greenwich time seemed the most logical over-all,
>> always available and correct. I was most bothered by a second added
>> onto every year, and that means I get older by the second annually.
>> Hmmmm - but then the need for calibrated timers for validation
>> purposes with complex testing? Is it the automated
>> stainers/processors they are worried about?
>> As for resetting timers to do staining, my eyes are the timer when
>> developing a chromogen during manual IHC procedures - this is
>> controlled with a microscope. I have let a clock run up in time to
>> garner a "ballpark" figure for optimal development, and found that
>> can vary from day to day too, not an exact timing to be sure.
>> I agree with Claire on silver staining, but depend on microscopic
>> examination to monitor silver (for finalizing GMS, Jones methenamine
>> silver) ,and other proper removal of dyes Luxol fast blue,
>> decolorizing tissue Gram stain.
>> Now for a question: Do you think CAP will ever require eye
>> calibration? Sorry to even suggest that -
>> Gayle Callis HTL, HT, MT(ASCP)
>> Research Histopathology Supervisor
>> Veterinary Molecular Biology
>> Montana State University
>> Bozeman MT 59717
>> At 01:02 PM 5/9/2007, you wrote:
>>> Why don't we all just get atomic clocks and be done with it. I don't
>>> believe they ever need to be calibrated. (unless the laws of physics
>>> and radioactive element half-lives suddenly change). I agree with
>>> Joe. Staining is a special talent anyway. I have had to reset timers
>>> to add more incubation time on stains lots of times (especially
>>> silver). Oh sorry, it's only Wednesday. Only two more days to go.
>>> Claire Ingles
>>> UW Hospital
>>> Madison WI
>>> From: email@example.com on behalf of Perry,
>>> Sent: Wed 5/9/2007 12:42 PM
>>> To: firstname.lastname@example.org
>>> Subject: [Histonet] timer calibration
>>> We calibrate our timers by doing the following. Use the telephone to
>>> call 303-499-7111. A voice will prompt you and tell you the time. At
>>> the minute turn on the timer and record the Coordinated Universal time
>>> and the timer time. Listen until the next minute and turn off the
>>> and record the Coordinated Universal Time and the time on the timer.
>>> Margaret Perry HT (ASCP)
>>> IHC Lab Manager Veterinary Science
>>> Animal Disease Research and Diagnostic Lab
>>> South Dakota State University
>>> Box 2175 North Campus Drive
>>> Brookings SD 57007
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