Re: [Histonet] Interesting discussion about RE: timer calibration

From:Kathleen Roberts

God forbid that you & your pathologist see the same color 
differently...I go through this with my boss every once in a while.  An 
H&E slide will look just fine to me, but to him it's too pink, whereupon 
he tells me to go clean my glasses, change out the H&E and restain the 
slide.  :oP  Fortunately, our lab is a university research lab, so we 
don't have to undergo CAP inspection.

Neurotoxicology Labs
Dept of Pharmacology & Toxicology
Rutgers University

Philip Oshel wrote:

> Sure, why not? Require all histotechs to pass a "color-blindness" 
> (note the quotes) eye exam. Given that women generally have better 
> color discrimination than men in the red-green end of the spectrum, 
> female histotechs should be more valuble and able to demand higher pay.
> I can just see medical supply houses all carrying Pantone color charts 
> ...
> Phil
>> I have followed this discussion with interest and a bit of humor on 
>> the rationale for this chore.  It escapes me a bit -  but I did like 
>> the idea of an atomic clock but then saw the reply about battery 
>> failure.  Also, Greenwich time seemed the most logical over-all, 
>> always available and correct.  I was most bothered by a second added 
>> onto every year, and that means I get older by the second annually. 
>> Hmmmm - but then the need for calibrated timers for validation 
>> purposes with complex testing?  Is it the automated 
>> stainers/processors they are worried about?
>> As for resetting timers to do staining, my eyes are the timer when 
>> developing a chromogen during manual IHC procedures - this is 
>> controlled with a microscope.  I have let a clock run up in time to 
>> garner a "ballpark" figure for optimal development, and found that 
>> can vary from day to day too, not an exact timing to be sure.
>> I agree with Claire on silver staining, but depend on microscopic 
>> examination to monitor silver (for finalizing GMS, Jones methenamine 
>> silver) ,and other proper removal of dyes  Luxol fast blue, 
>> decolorizing tissue Gram stain.
>> Now for a question:  Do you think CAP will ever require eye 
>> calibration?  Sorry to even suggest that -
>> Gayle Callis HTL, HT, MT(ASCP)
>> Research Histopathology Supervisor
>> Veterinary Molecular Biology
>> Montana State University
>> Bozeman MT 59717
>>    At 01:02 PM 5/9/2007, you wrote:
>>> Why don't we all just get atomic clocks and be done with it. I don't 
>>> believe they ever need to be calibrated. (unless the laws of physics 
>>> and radioactive element half-lives suddenly change).  I agree with 
>>> Joe. Staining is a special talent anyway. I have had to reset timers 
>>> to add more incubation time on stains lots of times (especially 
>>> silver). Oh sorry, it's only Wednesday. Only two more days to go.
>>> Claire Ingles
>>>   UW Hospital
>>> Madison WI
>>> ________________________________
>>> From: on behalf of Perry, 
>>> Margaret
>>> Sent: Wed 5/9/2007 12:42 PM
>>> To:
>>> Subject: [Histonet] timer calibration
>>> We calibrate our timers by doing the following.  Use the telephone to
>>> call 303-499-7111.  A voice will prompt you and tell you the time.  At
>>> the minute turn on the timer and record the Coordinated Universal time
>>> and the timer time.  Listen until the next minute and turn off the 
>>> timer
>>> and record the Coordinated Universal Time and the time on the timer.
>>> Margaret Perry HT (ASCP)
>>> IHC Lab Manager Veterinary Science
>>> Animal Disease Research and Diagnostic Lab
>>> South Dakota State University
>>> Box 2175 North Campus Drive
>>> Brookings SD 57007

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