Re: [Histonet] Gill's 3?

From:"Robyn Vazquez"

Is this the same with frozen sections too (4% acetic acid)?  I use a
linear stainer, so a 4% solution would be too strong for the length of
time it would be on the chain link.  I would be using a 30sec cup. 
Could I weaken the solution to accomadate the time?

>>> "Gayle Callis"  5/2/2007 12:48 PM >>>

No, is NOT regressive, Gill 3 is a progressive hematoxylin and it
from Gill 1 by having a higher concentration of hematoxylin (3X more)
the Gill 3.

Staining is usually done in approx 1 1/2 minutes, then you rinse with 
approx 10 dips or so 4% acetic acid only to get rid of ionic
interaction of 
hematoxylin to the glass slide and a bit of background staining in the

tissue.  The acetic acid is not a differentiation solution as is 1% HCl
70% alcohol (as used with Harris Hematoxylin).  I am not a fan of
anymore since it can no longer be made with mercuric oxide (boo hoo!) 
Harris tends to overstain, Gill will not do this.

We switched to Gill or other progressive hematoxylins years ago to get
from having to differentiate the Harris.

Simply, with Gill 3 you just stain, rinse with acetic, and blue in
tap water substitute.  Check out Richard Allan Gill hematoxylins, they
everything you need and the stain was superb.

Whoever wrote the Sigma tech sheet didn't know their hematoxylins IF
called Gill regressive!!  Gary Gill must be gagging over this one.

Gayle Callis (senior too!)

  At 12:23 PM 5/2/2007, you wrote:
>Excuse the question if it is patently obvious - perhaps I'm having a
>Senior Moment.  I occasionally do Congo Red (Sigma's kit) and it
>for Gill 3 Hematoxylin.  According to the description of Gill 3, it is
>regressive hematoxylin - as is Harris'.  Should I not have Gill 3 at
>that moment, what would be the result (pos/neg) of using Harris'?  A
>follow-on to this question: is there a vendor for Bouin's that sells
>less than a liter?  Thanks in advance.
>Sally Breeden, HT(ASCP)
>NM Dept. of Agriculture
>Veterinary Diagnostic Services
>PO Box 700
>Albuquerque, NM  87106
>Histonet mailing list

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610

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