Re: [Histonet] Fatty tissue

From:Rene J Buesa

Deep freeze quickly, cut slowly, increase thickness "just a little bit" and use the "anti-rolling" devise (of a camel hair brush) to "pull" the section.
  René J.

Jennifer Johnson  wrote:
  Does anyone have any suggestions for cutting better frozen sections of very
fatty tissue (breast in particular)? When I turn the cryostat colder, my
Pathologist complains about the artifact on the permanent sections. Any
help is appreciated. 



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