Start sectioning the block until you get to the are of interest ("face off"). Remove the block and place it "face down" on the softening liquid during 15 minutes. Cool it and try sectioning again.
  René J.

Anthony Gatt  wrote:
  I wrote recently asking for advice on sectioning drosophila
heads and many of you wrote back very useful advice
pertaining to softening the chitin before processing.
My question is...is there anything I can do for the 2 dozen
or so blocks that I had already processed and are waiting to be sectioned? These have NOT been processed with softeners but are important specimens.
Thanks

_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




       
---------------------------------
Take the Internet to Go: Yahoo!Go puts the Internet in your pocket: mail, news, photos & more. 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet