Someone on histonet mentioned a 15 second soak on your water bath, then back 
to the cold plate. It works wonders.



Sheila Adey HT MLT
Port Huron Hospital
Michigan





>From: LuAnn Anderson 
>To: "Mike Pence" ,"I-sanna Gibbons" 
>,
>Subject: RE: [Histonet] chattering
>Date: Fri, 11 May 2007 15:49:45 -0500
>
>I would soak the blocks, then make sure everything on the microtome is 
>tightened down well (blade, block clamp etc.) and see if that will work.
>
>
>At 03:33 PM 5/11/2007, Mike Pence wrote:
>>Chatter can be from a number of reasons.
>>
>>Loose knife blade.  Angle of knife not correct.  Too "dry" of tissue.
>>(over fixed)  Speed the tissue is cut across the blade.  The material
>>the tissue is embedded in.  The size of the tissue being cut.  C.L.
>>Sturkey, Inc.  Lebanon, PA. USA put out a paper about microtomy
>>trouble-shooting guide. See if you can locate that for reference.
>>
>>Mike
>>
>>-----Original Message-----
>>From: histonet-bounces@lists.utsouthwestern.edu
>>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of I-sanna
>>Gibbons
>>Sent: Friday, May 11, 2007 3:16 PM
>>To: histonet@lists.utsouthwestern.edu
>>Subject: [Histonet] chattering
>>
>>
>>Hi everyone, my problem is more chattering of the bat brain tissue
>>rather than cracking.  Does chattering occur easily with the bat brain?
>>Waht are the causes of this chattering and how can I prevent it?
>>   Thanks
>>   I-sanna
>>
>>   I-sanna Gibbons, DVM
>>   Veterinary Anatomy
>>   School of Veterinary Medicine
>>   The University of the West Indies
>>   Trinidad and Tobago, W.I.
>>
>>
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>>Visit the Yahoo! Auto Green Center.
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