I would soak the blocks, then make sure everything on the microtome 
is tightened down well (blade, block clamp etc.) and see if that will work.


At 03:33 PM 5/11/2007, Mike Pence wrote:
>Chatter can be from a number of reasons.
>
>Loose knife blade.  Angle of knife not correct.  Too "dry" of tissue.
>(over fixed)  Speed the tissue is cut across the blade.  The material
>the tissue is embedded in.  The size of the tissue being cut.  C.L.
>Sturkey, Inc.  Lebanon, PA. USA put out a paper about microtomy
>trouble-shooting guide. See if you can locate that for reference.
>
>Mike
>
>-----Original Message-----
>From: histonet-bounces@lists.utsouthwestern.edu
>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of I-sanna
>Gibbons
>Sent: Friday, May 11, 2007 3:16 PM
>To: histonet@lists.utsouthwestern.edu
>Subject: [Histonet] chattering
>
>
>Hi everyone, my problem is more chattering of the bat brain tissue
>rather than cracking.  Does chattering occur easily with the bat brain?
>Waht are the causes of this chattering and how can I prevent it?
>   Thanks
>   I-sanna
>
>   I-sanna Gibbons, DVM
>   Veterinary Anatomy
>   School of Veterinary Medicine
>   The University of the West Indies
>   Trinidad and Tobago, W.I.
>
>
>---------------------------------
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>Visit the Yahoo! Auto Green Center.
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