I've used the Abcam FOXP3 antibody in the past and had great results with it.  I pretty much just followed the data sheet that came with the antibody.  Good positive controls would be tonsil or lymphnode.

Tom Pier

>>> Gayle Callis  05/07/07 2:13 PM >>>
The technical data sheet should provide control information.  Spleen should 
work with this according to the eBiosciences data sheet/technical information.

At 12:57 PM 5/7/2007, you wrote:
>Thymus should be a good control for T cells.
>
>-----Original Message-----
>From: histonet-bounces@lists.utsouthwestern.edu
>[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Melissa
>Gonzalez
>Sent: Monday, May 07, 2007 11:36 AM
>To: histonet@lists.utsouthwestern.edu
>Subject: [Histonet] Regulatory Tcells (FoxP3) IHC
>
>Hi all,
>Anyone have any experience with this? How well does the Abcam antibody
>work on FFPE human tissues? What's a good positive control?
>Thanks
>Melissa

Gayle Callis HTL, HT, MT(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717


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