RE: [Histonet] RE: timer calibration

From:"Horn, Hazel V"



I think this is in the lab general checklist which applies to histology as well....

Hazel Horn
Hazel Horn, HT/HTL (ASCP)
Supervisor of Histology
Arkansas Children's Hospital
800 Marshall    Slot 820
Little Rock, AR   72202

phone   501.364.4240
fax        501.364.3912

visit us on the web at:    www.archildrens.org


-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Shimp, Kristen R
Sent: Thursday, May 10, 2007 12:57 PM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] RE: timer calibration

What CAP question number were you sited?  I haven't heard of this one pertaining to histology timers.
Kristen

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu]On Behalf Of
histonet-request@lists.utsouthwestern.edu
Sent: Wednesday, May 09, 2007 10:01 AM
To: histonet@lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 42, Issue 11


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Today's Topics:

   1. Placentas  (Cindy DuBois)
   2. protein block (Till, Renee)
   3. Re: protein block (Gayle Callis)
   4. timer calibration (Margiotta, Michele)
   5. If you wanted an antibody what would it be? (Patricia Adams)
   6. Re: timer calibration (Rene J Buesa)
   7. RE: [SPAM] [Histonet] reprocessing paraffin blocks
      (Mickie Johnson)
   8. RE: Regulatory Tcells (FoxP3) IHC (C.M. van der Loos)
   9. RE: Leishmania (C.M. van der Loos)
  10. FW: Her2 question (Cohen, Sherene B.)
  11. Re: FW: Her2 question (Rene J Buesa)
  12. Re: timer calibration (Joe Nocito)
  13. RE: timer calibration (Mighnon Lashus)
  14. RE: timer calibration (Douglas D Deltour)
  15. Alizarin Red staining for mineralization nodules (docqian)
  16. Leica ASP300 Tissue Processor for sale (Brian Branton)
  17. RE: timer calibration (Mitchell Jean A.)
  18. RE: timer calibration (Edwards, R.E.)
  19. RE: protein block (Patsy Ruegg)
  20. RE: timer calibration (Mike Pence)
  21. (no subject) (Kennedy, Lisa)
  22. Re: timer calibration (Bryan Hewlett)
  23. RE: (no subject) (Mike Pence)


----------------------------------------------------------------------

Message: 1
Date: Tue, 8 May 2007 10:27:35 -0700 (PDT)
From: Cindy DuBois 
Subject: [Histonet] Placentas 
To: Histonet 
Message-ID: <769673.20813.qm@web33409.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

We do this for one of our hospitals.  We do charge the patient / insurance =66or a "Gross Only" on the placentas we accession as a gross only specimen.  
   
  We were told that since the child  and / or parents can sue the hospital up to 20 years later for any perceived problems that may have arisen out of the pregnancy or labor, this was to cover the hospital legally.
   
  The only hassle we have with this is how to store all the blocks we are producing.
   
  Cindy DuBois
  Stockton, CA
   

       
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Message: 2
Date: Tue, 8 May 2007 13:36:38 -0500
From: "Till, Renee" 
Subject: [Histonet] protein block
To: histonet@lists.utsouthwestern.edu
Message-ID:
	<11F927674DEBDC43B960809A7403C5D204550AA5@MAILPED.ad.uams.edu>
Content-Type: text/plain; charset=us-ascii

I have a protocol from a paper that I need to try and reproduce. They used
the Protein Block from Dako, along with the LSAB+ kit. The Block seems to no
longer be available. What would be an alternative? Is this the same as the
serum block I would normally use? 

 

Renee' Till, HT

Research Assistant

Arkansas Children's Nutrition Center

1212 Marshall St./N2021

Little Rock, AR 72202

Lab (501)364-8504

Office Fax (501)364-3161

 


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------------------------------

Message: 3
Date: Tue, 08 May 2007 12:49:21 -0600
From: Gayle Callis 
Subject: Re: [Histonet] protein block
To: "Till, Renee" ,
	Histonet@lists.utsouthwestern.edu
Message-ID:
	<6.0.0.22.1.20070508124600.01b06170@gemini.msu.montana.edu>
Content-Type: text/plain; charset="us-ascii"; format=flowed

Renee,

I doubt it, sometimes protein blocks are serum-free blocks, and may contain=20
BSA and/or casein.  I would assume another company's block should do the 
job as well.  Try Lab Vision, Biocare, etc to see what they have available.

At 12:36 PM 5/8/2007, you wrote:
>I have a protocol from a paper that I need to try and reproduce. They used
>the Protein Block from Dako, along with the LSAB+ kit. The Block seems to no
>longer be available. What would be an alternative? Is this the same as the
>serum block I would normally use?
>
>

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610






------------------------------

Message: 4
Date: Tue, 8 May 2007 15:42:12 -0400
From: "Margiotta, Michele" 
Subject: [Histonet] timer calibration
To: 
Message-ID:
	<922CE5B88F398948B4076A9A4340E7AF036AF5FF@bmh_exchange.bmhmc.org>
Content-Type: text/plain; charset="iso-8859-1"

Hi All,

Does anyone have a procedure for calibrating timers? We just had an inspection and got cited because our timers were not calibrated.  Any info would be appreciated!

Michele



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Brookhaven Memorial Hospital Medical Center at (631) 654-7282.





------------------------------

Message: 5
Date: Tue, 8 May 2007 13:11:02 -0700 (PDT)
From: Patricia Adams 
Subject: [Histonet] If you wanted an antibody what would it be?
To: HistoNet 
Message-ID: <102360.41177.qm@web52512.mail.re2.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

First I wish to thank everyone for helping me
with my species antibody problems.

My question is for all of you other Vet School
and Vet clinic people.
Do you have problems finding certain types of
antibodies that will react on certain species, if
so what antibody and which species?

I am trying to compile a list of the most needed
antibodies that we need on the animal side of
things.
 
I do again wish to thank all who sent me help, I
have been burning up the Internet looking up all
the information sent my way.  And trying to dodge
my Pathologist and all his questions! (He knows
all my hiding places ;(, bummer!).

Patricia Adams
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------------------------------

Message: 6
Date: Tue, 8 May 2007 13:39:47 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] timer calibration
To: "Margiotta, Michele" ,
	histonet@pathology.swmed.edu
Message-ID: <113979.14134.qm@web61212.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Try any local watch repair store. They have to calibrate their repaired watches so it is very likely they can help you.
  Ren J.

"Margiotta, Michele"  wrote:
  Hi All,

Does anyone have a procedure for calibrating timers? We just had an inspection and got cited because our timers were not calibrated. Any info would be appreciated!

Michele



This e-mail and any files transmitted with it are confidential and are intended 
solely for the use of the individual or entity to which they are addressed.=20
This communication may contain material protected by the attorney-client 
privilege. If you are not the intended recipient or the person responsible =66or
delivering the e-mail to the intended recipient, be advised that you have received 
this e-mail in error and that any use, dissemination, forwarding, printing,=20
or copying of this e-mail is strictly prohibited. If you have received this e-mail in 
error, please immediately notify the sender via return e-mail or call
Brookhaven Memorial Hospital Medical Center at (631) 654-7282.



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 Check outnew cars at Yahoo! Autos.

------------------------------

Message: 7
Date: Tue, 8 May 2007 18:32:59 -0700
From: "Mickie Johnson" 
Subject: RE: [SPAM] [Histonet] reprocessing paraffin blocks
To: "'Gayle Callis'" ,
	
Message-ID:
	
	
Content-Type: text/plain;	charset="us-ascii"

Hi Everyone.  Thanks Gail for remembering my small article in Histo-Logic
about 5 years ago!

The procedure is very simple and the least harsh of any method I know. I did
not develop it, but one of my former students brought it back from NSH and
we used it with great success.

First, melt the block down and blot off the excess paraffin from the tissue.


Second, re-cassette the tissue in the same cassette, blotting excess
paraffin from the cassette.


Third, put the cassette in with the days normal tissue processing run, in
formalin.


Fourth, process as usual.

The next morning, embed and cut. The fat (or under processed tissue will cut
beautifully.

The rational is that the well processed part of the block will have paraffin
in it and will not feel the effects of dehydration. Xylene will melt out the
paraffin and then paraffin will re-infiltrate this part.

The unprocessed tissue areas are available to fix additionally and
dehydrate, clear and infiltrate with paraffin. 

The net result is a reprocessed block with no harsh treatment and very
little time expended to 'reprocess' by hand. It does take overnight to get
the slides, but usually the pathologist will be happy to see slides he can
read accurately.

Hope this helps.

Good Luck!

Mickie Johnson, B.S., HTL(ASCP)
Mohs Histology Consulting Services, LLC
  & Mohs Lab Staffing
2507 S. Manito Blvd.
Spokane, WA 99203
509-954-7134
Web: www.mohshistotemp.com & www.mohslabstaffing.com 
Email: mickie25@netzero.net
 
 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis
Sent: Friday, May 04, 2007 9:18 AM
To: Histonet@lists.utsouthwestern.edu
Subject: [SPAM] [Histonet] reprocessing paraffin blocks

Get this publication from Histo Logic on Sakura Finetek website.  It was 
very simple and very little work overall.

A=20
Technique for Correcting Poorly Processed Paraffin Blocks.
Michael L. Johnson, BS, HTL, HT(ASCP), Spokane, WA,  May 2003;XXXVI(1):21.

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610




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------------------------------

Message: 8
Date: Wed, 09 May 2007 09:19:03 +0200
From: "C.M. van der Loos" 
Subject: [Histonet] RE: Regulatory Tcells (FoxP3) IHC
To: histonet@lists.utsouthwestern.edu
Cc: Melissa.Gonzalez@cellgenesys.com, mauger@email.chop.edu
Message-ID: <132f4512faf0.12faf0132f45@amc.uva.nl>
Content-Type: text/plain; charset="us-ascii"


   Melissa,

   We  used  the  Abcam mouse antibody, clone 236A/E7 we great success on
   both  human  cryo  and  FFPE samples, and also in double staining with
   CD25,  CD4 (cryo).  For  more  details  please  see our paper recently
   accepted for JHC: Onno J. de Boer et al., Immunohistochemical Analysis
   of  Regulatory T Cell Markers FOXP3 and GITR on CD4^+CD25^+ T Cells in
   Normal  Skin  and  Inflammatory  Dermatoses.  Go  to  the  JHC website
   ([1]www.jhc.org) and go to exPRESS. You will find our paper under: May
   3rd 2007.

   Cheers,

   Chris van der Loos, PhD
   Dept. of Pathology
   Academic Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands

   Date: Mon, 7 May 2007 10:35:52 -0700
   From: "Melissa Gonzalez" 
   Subject: [Histonet] Regulatory Tcells (FoxP3) IHC
   To: 
   Message-ID:
   
   Content-Type: text/plain; charset="us-ascii"
   Hi all,
   Anyone have any experience with this? How well does the Abcam antibody
   work on FFPE human tissues? What's a good positive control?
   Thanks
   Melissa

References

   1. http://www.jhc.org/


------------------------------

Message: 9
Date: Wed, 09 May 2007 09:50:37 +0200
From: "C.M. van der Loos" 
Subject: [Histonet] RE: Leishmania
To: histonet@pathology.swmed.edu
Cc: SDrew@uwhealth.org
Message-ID: 
Content-Type: text/plain; charset="us-ascii"


   Dear Sally,

   Last  year  I  tried  an antibody from Cedarlane Leishmania LPG, clone
   CA7AE (1:500,  60  min,  RT).  I  could make it work with HIER citrate
   pH6.0 and indirect  fluorescence on FFPE samples. Spectral imaging was
   used for  unmixing  "real"  signal from autofluorescence. Even then it
   was  hard  to  find  the specific signal, due to some specific-looking
   nuclear  background  staining.  The  tissue samples that were positive
   with  fluorescence were also subjected to IHC with anti-mouse polymers
   and DAB or LPR as chromogens. This totally failed and we gave up.

   I realize it's not a very hopeful story but perhaps it helps anyway.

   Chris van der Loos, PhD
   Dept. of Pathology
   Academic Medical Center M2-230
   Meibergdreef 9
   NL-1105 AZ Amsterdam
   The Netherlands


   Date: Mon, 7 May 2007 15:03:28 -0500
   From: "Drew Sally A." 
   Subject: [Histonet] Leishmania
   To: "Histonet" 
   Could someone direct us to a place/person who might run an antibody
   against Leishmania?  We
   have a pathologist asking questions about it, and all our usual source
   don't list it.
   Thank you!
   Sally Ann Drew, MT(ASCP)
   IHC/ISH Laboratory
   University of Wisconsin Hosp. & Clinics
   Madison, WI 53792


------------------------------

Message: 10
Date: Wed, 9 May 2007 09:30:05 -0400 
From: "Cohen, Sherene B." 
Subject: [Histonet] FW: Her2 question
To: "'histonet@lists.utsouthwestern.edu'"
	
Message-ID:
	<670B8345AF238F40910FEC4CA7D4B7D2F9E991@exchserver.fccc.edu>
Content-Type: text/plain;	charset="iso-8859-1"



>  -----Original Message-----
> From: 	Cohen, Sherene B.  
> Sent:	Tuesday, May 08, 2007 3:12 PM
> To:	'histonet@lists.utsouthwestern.edu'
> Subject:	Her2 question
> 
> Hey netters,
> 
> My director just asked me about fixation in NBF and Her2.  I'm curious to
> know what others are doing about:
> 
> A)  Weekend processing of breast specimens
> 
> B)  If anyone has studied the antigenicity effect of these specimens
> sitting in warm paraffin for at least 8 hours.
> 
> Any feedeback is greatly appreciated.
> 
> Sherene
> 
> 



------------------------------

Message: 11
Date: Wed, 9 May 2007 07:35:01 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] FW: Her2 question
To: "Cohen, Sherene B." ,
	"'histonet@lists.utsouthwestern.edu'"
	
Message-ID: <222753.88085.qm@web61222.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

This was the subject of an extense discussion in Histonet just one month ago.
  It would be better for you to look in the archieves and benefit from that discussion.
  Ren J.

"Cohen, Sherene B."  wrote:
  

> -----Original Message-----
> From: Cohen, Sherene B. 
> Sent: Tuesday, May 08, 2007 3:12 PM
> To: 'histonet@lists.utsouthwestern.edu'
> Subject: Her2 question
> 
> Hey netters,
> 
> My director just asked me about fixation in NBF and Her2. I'm curious to
> know what others are doing about:
> 
> A) Weekend processing of breast specimens
> 
> B) If anyone has studied the antigenicity effect of these specimens
> sitting in warm paraffin for at least 8 hours.
> 
> Any feedeback is greatly appreciated.
> 
> Sherene
> 
> 

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Message: 12
Date: Mon, 14 May 2007 10:08:30 -0500
From: "Joe Nocito" 
Subject: Re: [Histonet] timer calibration
To: "Rene J Buesa" ,	"Margiotta, Michele"
	, 
Message-ID: <00c801c79639$bc0a91a0$d49eae18@yourxhtr8hvc4p>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=original

I'm sorry
am I the only one that thinks calibrating timers is stupid. I mean how many=20
histology procedures are so time sensitive that the timers have to be 
calibrated? Let's face it. I have had techs sit there and watch the clock=20
and rinse as soon as the timer goes off. And I've had techs wait for the 
timer to go off , then mosey over and rinse.  Both stains worked. I guess 
I'm just not anal enough.

JTT
----- Original Message ----- 
From: "Rene J Buesa" 
To: "Margiotta, Michele" ; 

Sent: Tuesday, May 08, 2007 3:39 PM
Subject: Re: [Histonet] timer calibration


> Try any local watch repair store. They have to calibrate their repaired 
> watches so it is very likely they can help you.
>  Ren J.
>
> "Margiotta, Michele"  wrote:
>  Hi All,
>
> Does anyone have a procedure for calibrating timers? We just had an 
> inspection and got cited because our timers were not calibrated. Any info=20
> would be appreciated!
>
> Michele
>
>
>
> This e-mail and any files transmitted with it are confidential and are 
> intended
> solely for the use of the individual or entity to which they are 
> addressed.
> This communication may contain material protected by the attorney-client
> privilege. If you are not the intended recipient or the person responsible=20
> for
> delivering the e-mail to the intended recipient, be advised that you have=20
> received
> this e-mail in error and that any use, dissemination, forwarding, 
> printing,
> or copying of this e-mail is strictly prohibited. If you have received 
> this e-mail in
> error, please immediately notify the sender via return e-mail or call
> Brookhaven Memorial Hospital Medical Center at (631) 654-7282.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ---------------------------------
> Ahhh...imagining that irresistible "new car" smell?
> Check outnew cars at Yahoo! Autos.
> _______________________________________________
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------------------------------

Message: 13
Date: Wed, 9 May 2007 10:18:51 -0500
From: "Mighnon Lashus" 
Subject: RE: [Histonet] timer calibration
To: 
Message-ID:
	<7DFAF4868AAAC34C986DF7E1AC16D02601156D54@pgnexchg1.pathgroup.com>
Content-Type: text/plain;	charset="iso-8859-1"

Who sited you?
Mighnon

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito
Sent: Monday, May 14, 2007 11:09 AM
To: Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu
Subject: Re: [Histonet] timer calibration

I'm sorry
am I the only one that thinks calibrating timers is stupid. I mean how many=20
histology procedures are so time sensitive that the timers have to be 
calibrated? Let's face it. I have had techs sit there and watch the clock=20
and rinse as soon as the timer goes off. And I've had techs wait for the 
timer to go off , then mosey over and rinse.  Both stains worked. I guess 
I'm just not anal enough.

JTT
----- Original Message ----- 
From: "Rene J Buesa" 
To: "Margiotta, Michele" ; 

Sent: Tuesday, May 08, 2007 3:39 PM
Subject: Re: [Histonet] timer calibration


> Try any local watch repair store. They have to calibrate their repaired 
> watches so it is very likely they can help you.
>  Ren J.
>
> "Margiotta, Michele"  wrote:
>  Hi All,
>
> Does anyone have a procedure for calibrating timers? We just had an 
> inspection and got cited because our timers were not calibrated. Any info=20
> would be appreciated!
>
> Michele
>
>
>
> This e-mail and any files transmitted with it are confidential and are 
> intended
> solely for the use of the individual or entity to which they are 
> addressed.
> This communication may contain material protected by the attorney-client
> privilege. If you are not the intended recipient or the person responsible=20
> for
> delivering the e-mail to the intended recipient, be advised that you have=20
> received
> this e-mail in error and that any use, dissemination, forwarding, 
> printing,
> or copying of this e-mail is strictly prohibited. If you have received 
> this e-mail in
> error, please immediately notify the sender via return e-mail or call
> Brookhaven Memorial Hospital Medical Center at (631) 654-7282.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ---------------------------------
> Ahhh...imagining that irresistible "new car" smell?
> Check outnew cars at Yahoo! Autos.
> _______________________________________________
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Message: 14
Date: Wed, 9 May 2007 11:25:31 -0500
From: "Douglas D Deltour" 
Subject: RE: [Histonet] timer calibration
To: "'Joe Nocito'" ,
	
Message-ID: <1033394974-763687002@pathology.swmed.edu>
Content-Type: text/plain;	charset="iso-8859-1"

Who inspects the calibrating timers? 
What are the calibrating timers calibrated against? What are the calibrating
timers of the calibrating timers calibrated against?  

 
Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
(803)252-1913
Fax (803)254-3262
 
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-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito
Sent: Monday, May 14, 2007 10:09 AM
To: Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu
Subject: Re: [Histonet] timer calibration

I'm sorry
am I the only one that thinks calibrating timers is stupid. I mean how many=20
histology procedures are so time sensitive that the timers have to be 
calibrated? Let's face it. I have had techs sit there and watch the clock=20
and rinse as soon as the timer goes off. And I've had techs wait for the 
timer to go off , then mosey over and rinse.  Both stains worked. I guess 
I'm just not anal enough.

JTT
----- Original Message ----- 
From: "Rene J Buesa" 
To: "Margiotta, Michele" ; 

Sent: Tuesday, May 08, 2007 3:39 PM
Subject: Re: [Histonet] timer calibration


> Try any local watch repair store. They have to calibrate their repaired 
> watches so it is very likely they can help you.
>  Ren J.
>
> "Margiotta, Michele"  wrote:
>  Hi All,
>
> Does anyone have a procedure for calibrating timers? We just had an 
> inspection and got cited because our timers were not calibrated. Any info=20
> would be appreciated!
>
> Michele
>
>
>
> This e-mail and any files transmitted with it are confidential and are 
> intended
> solely for the use of the individual or entity to which they are 
> addressed.
> This communication may contain material protected by the attorney-client
> privilege. If you are not the intended recipient or the person responsible

> for
> delivering the e-mail to the intended recipient, be advised that you have=20
> received
> this e-mail in error and that any use, dissemination, forwarding, 
> printing,
> or copying of this e-mail is strictly prohibited. If you have received 
> this e-mail in
> error, please immediately notify the sender via return e-mail or call
> Brookhaven Memorial Hospital Medical Center at (631) 654-7282.
>
>
>
> _______________________________________________
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>
>
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>
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------------------------------

Message: 15
Date: Wed, 9 May 2007 23:24:51 +0800 (CST)
From: docqian 
Subject: [Histonet] Alizarin Red staining for mineralization nodules
To: Histonet@lists.utsouthwestern.edu
Message-ID: <628956.75919.qm@web15210.mail.cnb.yahoo.com>
Content-Type: text/plain; charset=gb2312

Dear all,

I would like to perform Alizarin Red staining for mineralization nodules in primary osteoblasts, Does anyone have a protocol about it?
In addition, for mineralization nodule formation, we should add ascorbic acid and beta-glycerolphosphate into the culture medium, my question is :  use which solution to dissovle these drugs (distilled water, or culture medium).

Thank you.
 
Guofeng


      ___________________________________________________________ 
עŻ3.5G20M=B8=20
http://cn.mail.yahoo.com

------------------------------

Message: 16
Date: Wed, 9 May 2007 11:30:39 -0400
From: "Brian Branton" 
Subject: [Histonet] Leica ASP300 Tissue Processor for sale
To: 
Message-ID:
	<2A5183C7E289F646A4744C85841587BF04128A@timeclock.sarapath.com>
Content-Type: text/plain;	charset="iso-8859-1"

Hello Histonet,

We are selling one of our Leica ASP300 tissue processors. If anyone is interested, please checkout our eBay listing http://cgi.ebay.com/Leica-ASP300-Tissue-Processor_W0QQitemZ200106385547QQihZ010QQcategoryZ11816QQssPageNameZWDVWQQrdZ1QQcmdZViewItem.

Thank You

Brian Branton
Purchasing Agent
Sarasota Pathology
(941) 362-8963
(941) 362-8964 FAX




------------------------------

Message: 17
Date: Wed, 9 May 2007 10:45:00 -0500
From: "Mitchell Jean A." 
Subject: RE: [Histonet] timer calibration
To: 
Message-ID:
	<936BDBD9AB6ED84FB1FD25FD55DCDFB1035585A2@uwhis-xchng4.uwhis.hosp.wisc.edu>
	
Content-Type: text/plain;	charset="us-ascii"

JCAHO inspectors each and every time have inquired about my timer
calibrations.  I satisfy this requirement by annually calibrating my
timers to my wall clock; with the wall clocks calibrated to Greenwich
Mean Time (from a website)

Jean Mitchell, BS HT (ASCP)
University of Wisconsin Hospital & Clinics
Neuromuscular Laboratory Manager
600 Highland Avenue
Madison, WI  53792-5132 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Douglas
D Deltour
Sent: Wednesday, May 09, 2007 11:26 AM
To: 'Joe Nocito'; histonet@pathology.swmed.edu
Subject: RE: [Histonet] timer calibration


Who inspects the calibrating timers? 
What are the calibrating timers calibrated against? What are the
calibrating timers of the calibrating timers calibrated against?  

 
Douglas D. Deltour HT(ASCP)
Histology Manager
Professional Pathology Services, PC
One Science Court
Suite 200
Columbia, SC 29203
(803)252-1913
Fax (803)254-3262
 



------------------------------

Message: 18
Date: Wed, 9 May 2007 16:58:38 +0100
From: "Edwards, R.E." 
Subject: RE: [Histonet] timer calibration
To: "Joe Nocito" , "Rene J Buesa"
	,	"Margiotta, Michele" ,
	
Message-ID:
	
Content-Type: text/plain;	charset="iso-8859-1"

Calibration  of  timers  is  essential, as  one  of  the  least  publicised consequences of  global  warming  is  that  time  is  speeding  up  by about  a  second  a  year( Wells  et  al;Journal of  Man  and  Time Vol 6, 211-221, 2008). 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito
Sent: 14 May 2007 16:09
To: Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu
Subject: Re: [Histonet] timer calibration

I'm sorry
am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse.  Both stains worked. I guess I'm just not anal enough.

JTT
----- Original Message -----
From: "Rene J Buesa" 
To: "Margiotta, Michele" ; 
Sent: Tuesday, May 08, 2007 3:39 PM
Subject: Re: [Histonet] timer calibration


> Try any local watch repair store. They have to calibrate their 
> repaired watches so it is very likely they can help you.
>  Ren J.
>
> "Margiotta, Michele"  wrote:
>  Hi All,
>
> Does anyone have a procedure for calibrating timers? We just had an 
> inspection and got cited because our timers were not calibrated. Any 
> info would be appreciated!
>
> Michele
>
>
>
> This e-mail and any files transmitted with it are confidential and are 
> intended solely for the use of the individual or entity to which they 
> are addressed.
> This communication may contain material protected by the 
> attorney-client privilege. If you are not the intended recipient or 
> the person responsible for delivering the e-mail to the intended 
> recipient, be advised that you have received this e-mail in error and 
> that any use, dissemination, forwarding, printing, or copying of this 
> e-mail is strictly prohibited. If you have received this e-mail in 
> error, please immediately notify the sender via return e-mail or call 
> Brookhaven Memorial Hospital Medical Center at (631) 654-7282.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ---------------------------------
> Ahhh...imagining that irresistible "new car" smell?
> Check outnew cars at Yahoo! Autos.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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------------------------------

Message: 19
Date: Wed, 9 May 2007 10:34:31 -0600
From: "Patsy Ruegg" 
Subject: RE: [Histonet] protein block
To: "'Gayle Callis'" ,	"'Till, Renee'"
	, 
Message-ID: <002701c79257$ec227640$6501a8c0@Patsy>
Content-Type: text/plain;	charset="us-ascii"

Dako's protein block is serum free and from what I understand is just
casein, no bsa.  I use serum free protein block from Open Biosystems, I also
use their Stable Dab, Antibody Diluent, hematoxylin CS, etc., they are a lot
cheaper from most and I like their products.  
Patsy

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Gayle Callis
Sent: Tuesday, May 08, 2007 12:49 PM
To: Till, Renee; Histonet@lists.utsouthwestern.edu
Subject: Re: [Histonet] protein block

Renee,

I doubt it, sometimes protein blocks are serum-free blocks, and may contain=20
BSA and/or casein.  I would assume another company's block should do the 
job as well.  Try Lab Vision, Biocare, etc to see what they have available.

At 12:36 PM 5/8/2007, you wrote:
>I have a protocol from a paper that I need to try and reproduce. They used
>the Protein Block from Dako, along with the LSAB+ kit. The Block seems to
no
>longer be available. What would be an alternative? Is this the same as the
>serum block I would normally use?
>
>

Gayle Callis
MT,HT,HTL(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610




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------------------------------

Message: 20
Date: Wed, 9 May 2007 11:40:13 -0500
From: "Mike Pence" 
Subject: RE: [Histonet] timer calibration
To: "Edwards, R.E." , "Joe Nocito"
	,	"Rene J Buesa" ,	"Margiotta,
	Michele" , 
Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5C6@IS-E2K3.grhs.net>
Content-Type: text/plain;	charset="iso-8859-1"

If we are going to ask if our timers are calibrated in the Histology Lab, then one could ask the question: "Do you calibrate the timers on your various automated instruments such as processors, stainers, cryostats, and the list could go on?  I don't feel that 1 second a year is going to make a difference in my calibrated-timed tissue being processed or my H&E stains. If time does matter that precisely to you, then you most likely have a system in place for calibrating your timers.

Mike

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Edwards, R.E.
Sent: Wednesday, May 09, 2007 10:59 AM
To: Joe Nocito; Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu
Subject: RE: [Histonet] timer calibration


Calibration  of  timers  is  essential, as  one  of  the  least  publicised consequences of  global  warming  is  that  time  is  speeding  up  by about  a  second  a  year( Wells  et  al;Journal of  Man  and  Time Vol 6, 211-221, 2008). 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu [mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Joe Nocito
Sent: 14 May 2007 16:09
To: Rene J Buesa; Margiotta, Michele; histonet@pathology.swmed.edu
Subject: Re: [Histonet] timer calibration

I'm sorry
am I the only one that thinks calibrating timers is stupid. I mean how many histology procedures are so time sensitive that the timers have to be calibrated? Let's face it. I have had techs sit there and watch the clock and rinse as soon as the timer goes off. And I've had techs wait for the timer to go off , then mosey over and rinse.  Both stains worked. I guess I'm just not anal enough.

JTT
----- Original Message -----
From: "Rene J Buesa" 
To: "Margiotta, Michele" ; 
Sent: Tuesday, May 08, 2007 3:39 PM
Subject: Re: [Histonet] timer calibration


> Try any local watch repair store. They have to calibrate their
> repaired watches so it is very likely they can help you.
>  Ren J.
>
> "Margiotta, Michele"  wrote:
>  Hi All,
>
> Does anyone have a procedure for calibrating timers? We just had an
> inspection and got cited because our timers were not calibrated. Any 
> info would be appreciated!
>
> Michele
>
>
>
> This e-mail and any files transmitted with it are confidential and are
> intended solely for the use of the individual or entity to which they 
> are addressed.
> This communication may contain material protected by the 
> attorney-client privilege. If you are not the intended recipient or 
> the person responsible for delivering the e-mail to the intended 
> recipient, be advised that you have received this e-mail in error and 
> that any use, dissemination, forwarding, printing, or copying of this 
> e-mail is strictly prohibited. If you have received this e-mail in 
> error, please immediately notify the sender via return e-mail or call 
> Brookhaven Memorial Hospital Medical Center at (631) 654-7282.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ---------------------------------
> Ahhh...imagining that irresistible "new car" smell?
> Check outnew cars at Yahoo! Autos. 
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu 
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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------------------------------

Message: 21
Date: Wed, 9 May 2007 11:41:06 -0500
From: "Kennedy, Lisa" 
Subject: [Histonet] (no subject)
To: histonet@lists.utsouthwestern.edu
Message-ID:
	
	
Content-Type: text/plain; charset=iso-8859-1

I am doing a search for information regarding Histologists who perform grossing in and dictation of surgical specimens.  We have, in the past couple of years, begun to do that at our facility and are trying to get an idea of the increase of pay for histologist that doing this job warrants.  Can anyone out there give me some pay ranges, etc. for Histologists who perform these tasks.  Thanks so much.
Lisa Kennedy, HT(ASCP)
Good Samaritan Hospital
Kearney, NE  68847




------------------------------

Message: 22
Date: Wed, 9 May 2007 12:43:11 -0400
From: "Bryan Hewlett" 
Subject: Re: [Histonet] timer calibration
To: "Joe Nocito" , "Rene J Buesa"
	,	"Margiotta, Michele" ,
	
Message-ID: <002d01c79259$22348380$6500a8c0@mainbox>
Content-Type: text/plain; format=flowed; charset="iso-8859-1";
	reply-type=response

Joe,

You are talking fundamental philosophical differences here.
The inspectors are obviously Newtonian realists, while you're leaning more=20
to the Leibnitz/Kant view.
They are going to win!

Bryan

----- Original Message ----- 
From: "Joe Nocito" 
To: "Rene J Buesa" ; "Margiotta, Michele" 
; 
Sent: Monday, May 14, 2007 11:08 AM
Subject: Re: [Histonet] timer calibration


I'm sorry
am I the only one that thinks calibrating timers is stupid. I mean how many
histology procedures are so time sensitive that the timers have to be
calibrated? Let's face it. I have had techs sit there and watch the clock
and rinse as soon as the timer goes off. And I've had techs wait for the
timer to go off , then mosey over and rinse.  Both stains worked. I guess
I'm just not anal enough.

JTT
----- Original Message ----- 
From: "Rene J Buesa" 
To: "Margiotta, Michele" ;

Sent: Tuesday, May 08, 2007 3:39 PM
Subject: Re: [Histonet] timer calibration


> Try any local watch repair store. They have to calibrate their repaired 
> watches so it is very likely they can help you.
>  Ren J.
>
> "Margiotta, Michele"  wrote:
>  Hi All,
>
> Does anyone have a procedure for calibrating timers? We just had an 
> inspection and got cited because our timers were not calibrated. Any info=20
> would be appreciated!
>
> Michele
>
>
>
> This e-mail and any files transmitted with it are confidential and are 
> intended
> solely for the use of the individual or entity to which they are 
> addressed.
> This communication may contain material protected by the attorney-client
> privilege. If you are not the intended recipient or the person responsible=20
> for
> delivering the e-mail to the intended recipient, be advised that you have=20
> received
> this e-mail in error and that any use, dissemination, forwarding, 
> printing,
> or copying of this e-mail is strictly prohibited. If you have received 
> this e-mail in
> error, please immediately notify the sender via return e-mail or call
> Brookhaven Memorial Hospital Medical Center at (631) 654-7282.
>
>
>
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
> ---------------------------------
> Ahhh...imagining that irresistible "new car" smell?
> Check outnew cars at Yahoo! Autos.
> _______________________________________________
> Histonet mailing list
> Histonet@lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet


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------------------------------

Message: 23
Date: Wed, 9 May 2007 11:50:35 -0500
From: "Mike Pence" 
Subject: RE: [Histonet] (no subject)
To: "Kennedy, Lisa" ,
	
Message-ID: <661949901A768E4F9CC16D8AF8F2838CA1C5C7@IS-E2K3.grhs.net>
Content-Type: text/plain;	charset="us-ascii"

This is going to depend on the degree of grossing the tech does.  If
they only put GI type bx's in or if they do the entire gross-in of
specimens to the degree a PA or Resident does.  You may get a wide range
of pay.

Mike

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Kennedy,
Lisa
Sent: Wednesday, May 09, 2007 11:41 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] (no subject)


I am doing a search for information regarding Histologists who perform
grossing in and dictation of surgical specimens.  We have, in the past
couple of years, begun to do that at our facility and are trying to get
an idea of the increase of pay for histologist that doing this job
warrants.  Can anyone out there give me some pay ranges, etc. for
Histologists who perform these tasks.  Thanks so much. Lisa Kennedy,
HT(ASCP) Good Samaritan Hospital Kearney, NE  68847


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------------------------------

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