RE: [Histonet] PSR

From:"Patsy Ruegg"

When I did picro sirrus I rinsed well in dih20 to clear the yellow picric
acid, then airdryed and coverslipped with permount.

-----Original Message-----
[] On Behalf Of Molinari,
Sent: Thursday, May 03, 2007 4:17 AM
Subject: RE: [Histonet] PSR 

Thanks Gail I will give it a go.

Betsy Molinari HT (ASCP)
Texas Heart Institute
Cardiovascular Pathology
6770 Bertner Ave.
Houston,TX 77030
832-355-6812 (fax)

-----Original Message-----
From: Gayle Callis [] 
Sent: Wednesday, May 02, 2007 2:51 PM
To: Molinari, Betsy
Subject: Re: [Histonet] PSR 


After staining, try blotting the slide to dry, then coverslip without
through the alcohols, etc - just like van Giesons.

Gayle Callis

At 12:41 PM 5/2/2007, you wrote:
>After coverslipping, my picro Sirius red slides show a leaching of what
>seems to be the picric acid. I see a "halo" of yellow . I have tried
>extending the acidified water rinses and followed w/ 100% ETOH - Xylene
>. Any ideas?

Gayle Callis
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University - Bozeman
PO Box 173610
Bozeman MT 59717-3610

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