I believe this calibration has been on the general check list since back in the 80s. I remember how silly we thought it was, most inspectors don't pay attention in AP. I think it is more critical in the clinical lab.
Saint Joseph's Hospital
5665 Peachtree Dunwoody Rd NE
Atlanta, GA 30342
404-851-7376 - Phone
404-851-7831 - Fax
[mailto:firstname.lastname@example.org]On Behalf Of Gayle
Sent: Wednesday, May 09, 2007 3:56 PM
To: Ingles Claire; Histonet@lists.utsouthwestern.edu
Subject: [Histonet] Interesting discussion about RE: timer calibration
I have followed this discussion with interest and a bit of humor on the
rationale for this chore. It escapes me a bit - but I did like the idea
of an atomic clock but then saw the reply about battery failure. Also,
Greenwich time seemed the most logical over-all, always available and
correct. I was most bothered by a second added onto every year, and that
means I get older by the second annually. Hmmmm - but then the need for
calibrated timers for validation purposes with complex testing? Is it the
automated stainers/processors they are worried about?
As for resetting timers to do staining, my eyes are the timer when
developing a chromogen during manual IHC procedures - this is controlled
with a microscope. I have let a clock run up in time to garner a
"ballpark" figure for optimal development, and found that can vary from day
to day too, not an exact timing to be sure.
I agree with Claire on silver staining, but depend on microscopic
examination to monitor silver (for finalizing GMS, Jones methenamine
silver) ,and other proper removal of dyes Luxol fast blue, decolorizing
tissue Gram stain.
Now for a question: Do you think CAP will ever require eye
calibration? Sorry to even suggest that -
Gayle Callis HTL, HT, MT(ASCP)
Research Histopathology Supervisor
Veterinary Molecular Biology
Montana State University
Bozeman MT 59717
At 01:02 PM 5/9/2007, you wrote:
>Why don't we all just get atomic clocks and be done with it. I don't
>believe they ever need to be calibrated. (unless the laws of physics and
>radioactive element half-lives suddenly change). I agree with Joe.
>Staining is a special talent anyway. I have had to reset timers to add
>more incubation time on stains lots of times (especially silver). Oh
>sorry, it's only Wednesday. Only two more days to go.
> UW Hospital
>From: email@example.com on behalf of Perry, Margaret
>Sent: Wed 5/9/2007 12:42 PM
>Subject: [Histonet] timer calibration
>We calibrate our timers by doing the following. Use the telephone to
>call 303-499-7111. A voice will prompt you and tell you the time. At
>the minute turn on the timer and record the Coordinated Universal time
>and the timer time. Listen until the next minute and turn off the timer
>and record the Coordinated Universal Time and the time on the timer.
>Margaret Perry HT (ASCP)
>IHC Lab Manager Veterinary Science
>Animal Disease Research and Diagnostic Lab
>South Dakota State University
>Box 2175 North Campus Drive
>Brookings SD 57007
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