RE: [Histonet] GILL 3 Confusion


And yet on the two preceding pages, Gill 1 and Gill 2 are both described as progressive.  Both Bancroft and Luna describe them as being regressive.  Odd isn't it?  I had always been taught that all Gill hematoxylin are progressive.

I use my Sigma Gill 3 progressively, but I'm quite sure you could use it regressively if your procedure would allow it.  I also use Harris' progressively and regressively some of my other procedures as well.  I believe your choice of hematoxylins is all in how you want to use them and how you want your end product to look.

For our Congo Red's we use Harris' regressively before the CR solutions.

Kathy Johnston
Tech II Special Stains - Anatomic Pathology
Calgary Laboratory Services
#9 - 3535 Research Road NW
Calgary, AB
403-770-3572 phone
403-770-3731 fax

-----Original Message-----
[]On Behalf Of Breeden,
Sent: May 2, 2007 1:16 PM
Subject: [Histonet] GILL 3 Confusion

I'm making this follow-up post because I'm getting some conflicting
information about Gill 3 Hematoxylin.  Quoting from the Sigma 2006-2007
Biochemicals, Reagents & Kits catalog, page 1196:  "General purpose
nuclear stain, regressive type. Used with hematoxylin and eosin
staining."  Thus my question about substituting Harris' for Gill 3.
I've had a couple responses telling me that Gill 3 is PROGRESSIVE.  So,


Sally Breeden, HT(ASCP)

NM Dept. of Agriculture

Veterinary Diagnostic Services

PO Box 700

Albuquerque, NM  87106



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