Hello, I am currently sectioning drosophila heads and am tearing up the paraffin. Without the heads, I get beautiful ribbons so I suspect it is the sample itself. I am using a tissue processor with the following protocol after an o/n fix in 4% PFA.
15m -alcoholic formalin (x2)
30m -95% EtOH (x2)
30m -100% EtOH (x2)
30m -Histoclear (x3)
30m -Paraffin (x2)
Embed immediately in paraffin, cold block 30m, section next day.
I am new to this an would greatly appreciate any help.
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