dear histonetters
   
  hope atleast the persons remember my problem of alcian blue(sigma) precpitating in dis. water.
   
  I went on to stirr for 2 hours followed by boiling for 5 min. I could make a 2.5ph solution of alcian blue and its staining well. I think boiling was the main reason. 
   
  thanks for all those suggestions
   
  raghul
  medclone biotech
  chennai, india

       
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