[Histonet] Re: Histonet Digest, Vol 42, Issue 2

From:annamaria touvra



Hello There!
  I am interested too for this article. Any protocols are welcome, 
  Thank you,
  Anna-Maria
   
  Nicosia, RF and Ottinetti A. 
Modulation of microvascular growth and morphogenesis by reconstituted 
basement membrane gel in three dimensional cultures of rat aorta: a 
comparative study of angiogenesis in matrigel,
collagen, fibrin, and plasma clot.
In Vitro Cell Dev Biol. 1990 Feb;26(2):119-28.



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Today's Topics:

1. California Society for Histotechnology Spring Meeting May
17-20 (Morken, Tim)
2. Cell Button Prep (Heckford, Karen - SMMC-SF)
3. bone marrow smears (path lab)
4. Fatty tissue (Jennifer Johnson)
5. 2 F/T Permanent Histology Bench and Histo Supv positions in
SW Texas & East Texas (Eric Dye (ext 223))
6. peloris? (connie grubaugh)
7. Certified thermometers (WWmn916@aol.com)
8. RE: Cell Button Prep (Kemlo Rogerson)
9. osteoclast/ osteoblast markers (louise renton)
10. CD52 (Doug Geddes)
11. alcian blue_precipitaion_solution (raghul)
12. Re: Fatty tissue (Rene J Buesa)
13. MOHS (April Sachau)
14. Re: Certified thermometers (Rene J Buesa)
15. Re: MOHS (Rene J Buesa)
16. looking for Freida Carson (godsgalnow@aol.com)
17. RE: Certified thermometers (Kemlo Rogerson)
18. RE: Certified thermometers (Rene J Buesa)
19. RE: Certified thermometers (soofia siddiqui)
20. Aortic ring references (Andrea T. Hooper)


----------------------------------------------------------------------

Message: 1
Date: Tue, 1 May 2007 14:22:46 -0400
From: "Morken, Tim" 
Subject: [Histonet] California Society for Histotechnology Spring
Meeting May 17-20 
To: "histonet" 
Message-ID:
<6BFF6D137DF6BC43B33891BA96E83B1959B5DD@PGHCR-EXMB-VS-1.na.fshrnet.com>

Content-Type: text/plain; charset="us-ascii"

Information and registration forms for the California Society for
Histotechnology Spring Meeting on May 17-20 in San Mateo (near San Jose)
can be downloaded at the California society website:

www.californiahistology.org


Tim Morken






------------------------------

Message: 2
Date: Tue, 1 May 2007 12:51:36 -0700 
From: "Heckford, Karen - SMMC-SF" 
Subject: [Histonet] Cell Button Prep
To: "Histonet (histonet@lists.utsouthwestern.edu)"

Message-ID: 
Content-Type: text/plain; charset="us-ascii"

I was wondering if anyone would be so kind as giving input on the different
ways of preparing a cell button. We work a lot with Pleural Fluid and
Ascites Fluid. I have been told two different ways. One is just 1/2 and
1/2 with fluid and 95%alcohol and spend down decant and get your cell
button. The other is spend down the fluid decant add 10%formalin respin and
let set then get your cell button. Of course this all after I have made my
cytospins. We do not have a ThinPrep type machine. Any input would be very
appreciative. I realize there is probably more ways to do this that I have
not heard of.
Cheers,

Karen Heckford HT (ASCP) CE
Lead Histology Technician
Histology/Pathology Department
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
Fax: 415-750-8123
email: kheckfor@chw.edu

------------------------------

Message: 3
Date: Tue, 1 May 2007 16:24:42 -0400
From: "path lab" 

Subject: [Histonet] bone marrow smears
To: histonet@lists.utsouthwestern.edu
Message-ID:
<73841640705011324w79dfab34u57e8553b37923836@mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Could anyone please provide a procedure for bone marrow smears from necropsy
to staining with Wrights-Giemsa?

Thanks for your help in advance.


------------------------------

Message: 4
Date: Tue, 1 May 2007 16:55:49 -0400
From: "Jennifer Johnson" 
Subject: [Histonet] Fatty tissue
To: 
Message-ID: <000701c78c33$1eb04e00$2082010a@main.crispregional.org>
Content-Type: text/plain; charset="us-ascii"

Does anyone have any suggestions for cutting better frozen sections of very
fatty tissue (breast in particular)? When I turn the cryostat colder, my
Pathologist complains about the artifact on the permanent sections. Any
help is appreciated. 

Thanks,

Jennifer



------------------------------

Message: 5
Date: Tue, 1 May 2007 18:18:17 -0400
From: Eric Dye (ext 223) 
Subject: [Histonet] 2 F/T Permanent Histology Bench and Histo Supv
positions in SW Texas & East Texas
To: Histonetters 
Message-ID: 
Content-Type: text/plain

Hi - Histonetters -Are you still working at ?

I am presently on a search for several of my clients in Texas who are looking to hire HistoTech employees. I have Two(2) F/T Permanent Histology positions in Texas.BOTH positions are perm F/T as direct employees of my clients. They are regular employee positions that offer you 40hrs/wk with full employe benfits - including: 401-K, Full Health Insur., Vacation/Sick/Holiday & Full Relo.

Permanent Histology positions in Texas
1. S.W. Texas - Bench Histo Tech - Mon - Fri Days - work schedule is flexible to meet YOUR needs. 
2. East Texas - HistoTech Supervisor - Mon - Fri Days - 8a -5pm 
3. East Texas - Bench Histo Tech Supervisor - Mon - Fri Days - hours flex to suit your needs.

If you are interested in any of the HISTOTECH J0BS listed above - please call me at 800-466-9919 ext 223 or cell - 407-756-5507...OR ..
May I call you at or to discuss these Histo Tech openings?

Thank you, 

Eric Dye-Sr Allied Healthcare Recruiter
800-466-9919 ext 223 or Cell - 407-756-5507 

P.S.: Feel free to pass along this email and my phone number to anyone who you think might be interested. 
P.S.S.: The clients are currently interviewing - and the positions will close soon - so if you are interested, please call me today at 1-800-466-9919 ext 223 ---------------------------------------------------------------
Ategra Systems Inc
Specialists in Permanent & Contract Staffing
800-466-9919 ext 223 - voice
407 671 6075 - fax
eric@ategra.com
To Learn More About Ategra:
http://www.ategra.com 
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------------------------------

Message: 6
Date: Wed, 2 May 2007 03:19:58 +0000
From: connie grubaugh 
Subject: [Histonet] peloris?
To: "histonet@lists.utsouthwestern.edu"

Message-ID: 
Content-Type: text/plain; charset="Windows-1252"




In the market for a new processor and would like feed back on the Peloris good and bad from anyone that has this machine.
Connie G.
_________________________________________________________________
Discover the new Windows Vista
http://search.msn.com/results.aspx?q=windows+vista&mkt=en-US&form=QBRE

------------------------------

Message: 7
Date: Tue, 1 May 2007 23:33:21 EDT
From: WWmn916@aol.com
Subject: [Histonet] Certified thermometers
To: histonet@pathology.swmed.edu
Message-ID: 
Content-Type: text/plain; charset="US-ASCII"

Hello everyone,
I'm hoping for a little help. What is everyone using to calibrate routine 
thermometers in the histology lab (thermometers for refrig, H20 baths, IPOX 
machines....etc.)? We can no longer use mercury filled certified thermometers to 
test the others. Any suggestions? How often do the certified thermometers 
need to be recalibrated?

Thanks much,
Deb King
California





************************************** See what's free at http://www.aol.com.


------------------------------

Message: 8
Date: Wed, 2 May 2007 08:11:00 +0100
From: "Kemlo Rogerson" 
Subject: RE: [Histonet] Cell Button Prep
To: "Heckford, Karen - SMMC-SF" ,

Message-ID:
<86ADE4EB583CE64799A9924684A0FBBF01247645@wahtntex2.waht.swest.nhs.uk>
Content-Type: text/plain; charset="us-ascii"

You could try the hypaque method. If you are interested I could get the
method but it bluntly uses a density gradient. You spin down the fluid
remove supernatant and reconstitute with saline. You make a solution of
hypaque and layer the cell/ saline solution on top. You then spin, rbcs
and crude fall through the hypaque and larger cells float on top of the
hypaque. Harvest these cells, wash and then mix with agar and cool.
Process and cut and stain. Job done.

But ThinPrep is the way forward from my experience and if you are clever
you can come up with some sedimentation process that might emulate
ThinPrep similar to the one SurgiPath was flogging; I think it was
French but god knows what happened to it. 

Kemlo Rogerson
Pathology Manager
DD 01934 647057 or extension 3311
Mob 07749 754194; Pager 07659 597107;

We need to be open to the possibility that colleagues and even strangers
have information and perspectives that may be of value to us. --Margaret
Wheatley 

This e-mail is confidential and privileged. If you are not the intended
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Please inform me that this message has gone astray before deleting it.
Thank you for your co-operation





------------------------------

Message: 9
Date: Wed, 2 May 2007 10:38:18 +0200
From: "louise renton" 
Subject: [Histonet] osteoclast/ osteoblast markers
To: Histonet@lists.utsouthwestern.edu
Message-ID:

Content-Type: text/plain; charset=ISO-8859-1; format=flowed

Hi all,

what recommendations for the most specific immuno markers for
osteoclasts, osteoblasts and macrophages in FFPE primate/human tissue?
Anybody using CD163 and what have your results been like

Thanks in advance

best regards

-- 
Louise Renton
Bone Research Unit
University of the Witwatersrand
Johannesburg
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin
No trees were killed in the sending of this message.
However, many electrons were terribly inconvenienced.


------------------------------

Message: 10
Date: Wed, 02 May 2007 08:04:42 -0400
From: "Doug Geddes" 
Subject: [Histonet] CD52
To: 
Message-ID: <4638461A0200006100001DF6@lhscgwiao.lhsc.on.ca>
Content-Type: text/plain; charset=US-ASCII

Looking for a reliable supplier of CD52 for formalin fixed paraffin
embedded tissue, and any methods.




Doug Geddes BSc, MLT
Department of Pathology
London Helath Sciences Centre
London, ON
Canada




------------------------------

Message: 11
Date: Wed, 2 May 2007 14:32:15 +0100 (BST)
From: raghul 
Subject: [Histonet] alcian blue_precipitaion_solution
To: histonet@lists.utsouthwestern.edu
Message-ID: <681959.20532.qm@web8322.mail.in.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

dear histonetters

hope atleast the persons remember my problem of alcian blue(sigma) precpitating in dis. water.

I went on to stirr for 2 hours followed by boiling for 5 min. I could make a 2.5ph solution of alcian blue and its staining well. I think boiling was the main reason. 

thanks for all those suggestions

raghul
medclone biotech
chennai, india


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------------------------------

Message: 12
Date: Wed, 2 May 2007 06:40:26 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] Fatty tissue
To: Jennifer Johnson ,
histonet@lists.utsouthwestern.edu
Message-ID: <885157.50026.qm@web61219.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Deep freeze quickly, cut slowly, increase thickness "just a little bit" and use the "anti-rolling" devise (of a camel hair brush) to "pull" the section.
Ren J.

Jennifer Johnson wrote:
Does anyone have any suggestions for cutting better frozen sections of very
fatty tissue (breast in particular)? When I turn the cryostat colder, my
Pathologist complains about the artifact on the permanent sections. Any
help is appreciated. 

Thanks,

Jennifer

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Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





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------------------------------

Message: 13
Date: Wed, 2 May 2007 08:40:32 -0500
From: "April Sachau" 
Subject: [Histonet] MOHS
To: "raghul" ,

Message-ID:
<7E3ACD48BA6E26408F3188FBF08693F78B246F@titansbs1.corp.titanmed.com>
Content-Type: text/plain; charset="us-ascii"

Good Morning,
Can anyone please tell me why it has been impossible to find someone
with strong MOHS experience?


April Sachau
Titan Medical Group
Staff Supervisor
Phone (866) 332-9600 Ext. 1023
Fax (402) 332-5181
asachau@titanmed.com 

see us on the web at www.titanmed.com

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of raghul
Sent: Wednesday, May 02, 2007 8:32 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] alcian blue_precipitaion_solution

dear histonetters

hope atleast the persons remember my problem of alcian blue(sigma)
precpitating in dis. water.

I went on to stirr for 2 hours followed by boiling for 5 min. I could
make a 2.5ph solution of alcian blue and its staining well. I think
boiling was the main reason. 

thanks for all those suggestions

raghul
medclone biotech
chennai, india


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------------------------------

Message: 14
Date: Wed, 2 May 2007 06:50:36 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] Certified thermometers
To: WWmn916@aol.com, histonet@pathology.swmed.edu
Message-ID: <872229.17519.qm@web61224.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Calibrate them yourself! Just place your thermometer in boilong water and, if you are at sea level and there is no atmosferic low pressure or metheorological disturbance around, your thermometer should read 100C when the water starts to boil. If there is a different reading (either higher or lower) that difference will be your thermometer correction, meaning that to whatever reading you have, you will have to add (+) or substract (-) that difference.
For 0C fill a container with distilled water and add ice cubes and, with the same provisions as before, the reading should be 0C when there is a surface ice crust of ice in the water + ice.
Once a year is enough OR you could buy a digital thermometer (for less that $35). ALL come certified and avoid all the hassle previously described!
Ren J.

WWmn916@aol.com wrote:
Hello everyone,
I'm hoping for a little help. What is everyone using to calibrate routine 
thermometers in the histology lab (thermometers for refrig, H20 baths, IPOX 
machines....etc.)? We can no longer use mercury filled certified thermometers to 
test the others. Any suggestions? How often do the certified thermometers 
need to be recalibrated?

Thanks much,
Deb King
California





************************************** See what's free at http://www.aol.com.
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet





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------------------------------

Message: 15
Date: Wed, 2 May 2007 07:03:17 -0700 (PDT)
From: Rene J Buesa 
Subject: Re: [Histonet] MOHS
To: April Sachau , raghul
, histonet@lists.utsouthwestern.edu
Message-ID: <825625.80927.qm@web61214.mail.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

In the last issue of Advance MLP comes an address that you could try:
www.mohshistotemp.com 
Ren J.

April Sachau wrote:
Good Morning,
Can anyone please tell me why it has been impossible to find someone
with strong MOHS experience?


April Sachau
Titan Medical Group
Staff Supervisor
Phone (866) 332-9600 Ext. 1023
Fax (402) 332-5181
asachau@titanmed.com 

see us on the web at www.titanmed.com

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of raghul
Sent: Wednesday, May 02, 2007 8:32 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] alcian blue_precipitaion_solution

dear histonetters

hope atleast the persons remember my problem of alcian blue(sigma)
precpitating in dis. water.

I went on to stirr for 2 hours followed by boiling for 5 min. I could
make a 2.5ph solution of alcian blue and its staining well. I think
boiling was the main reason. 

thanks for all those suggestions

raghul
medclone biotech
chennai, india


---------------------------------
Check out what you're missing if you're not on Yahoo! Messenger 
_______________________________________________
Histonet mailing list
Histonet@lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


_______________________________________________
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet





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------------------------------

Message: 16
Date: Wed, 02 May 2007 10:05:14 -0400
From: godsgalnow@aol.com
Subject: [Histonet] looking for Freida Carson
To: histonet@lists.utsouthwestern.edu
Message-ID: <8C95ADA0A9C8D49-D0C-449A@FWM-R13.sysops.aol.com>
Content-Type: text/plain; charset="us-ascii"

I am looking for Freida...can you please email me.

Thanks,
Roxanne Soto HT(ASCP)QIHC
________________________________________________________________________
AOL now offers free email to everyone. Find out more about what's free from AOL at AOL.com.


------------------------------

Message: 17
Date: Wed, 2 May 2007 15:59:29 +0100
From: "Kemlo Rogerson" 
Subject: RE: [Histonet] Certified thermometers
To: "Rene J Buesa" , ,

Message-ID:
<86ADE4EB583CE64799A9924684A0FBBF01247657@wahtntex2.waht.swest.nhs.uk>
Content-Type: text/plain; charset="Windows-1252"

your thermometer should read 100C when the water starts to boil.
Rene

If water boils longer does it get hotter? I thought water boiled at 100 degrees however long you left it; if you increased the heat it still boiled at 100 degrees but the increased latent heat of vaporisation meant that more water was able to vaporise but still only at 100 degrees.
Kemlo

For 0C fill a container with distilled water and add ice cubes and, with the same provisions as before, the reading should be 0C when there is a surface ice crust of ice in the water + ice.
Rene

Same with freezing; I thought water could be 'frozen' at the eutectic point of water which is +4 degrees C (that's how you get 'black ice'). It certainly is at its most dense (which is why ice floats) and wouldn't the bottom of the water be hotter than at the top cos the coldest water floats (its less dense at the eutectic point) that's why we are here are were able to crawl out of the sea and my Koi don't freeze in Winter. Never really understood water that why I used to brew beer in it. I'd buy a digital thermometer because those of you at sea level will soon drown anyway with global warming.

=== message truncated ===


~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~ 
Anna-Maria Touvra MSc 
Democritus University of Thrace 
Department of Physical Education and Sport Science 
Address: University Campus, TEFAA Komotini 69100, GREECE 
Tel: 00306938511677, 00302531030343 

Contact information in Finland 
Address: Haperontie 1B 31, 40640 JYVSKYL, FINLAND 
Tel: 00358509324688 
~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~~
       
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