What type of paraffin are you using? We had similar problems awhile
back. The paraffin we had used for many years had a change in where is
was being manufactured, which resulted in somewhat of a different
recipe. Call me and I can fill you in on what we were using and how we
fixed the problem.
Anatomic Pathology Manager
Lower Columbia Pathologists
1217 14th Ave
Longview, WA 98632
[mailto:firstname.lastname@example.org] On Behalf Of Scott,
Sent: Friday, May 11, 2007 9:43 AM
Subject: [Histonet] FW: H&E Issues
> -----Original Message-----
> From: Scott, Allison D
> Sent: Friday, May 11, 2007 11:31 AM
> To: 'email@example.com'
> Subject: H&E Issues
> We are having a problem with our H&E slides. The problem just started
this week. One of our pathologist said that the stain was too dark.
After looking at the solutions in the stainer, I determined that the
bluing solution had not been changed as it should have been and I
changed it. I also changed the hematoxylin and the clarifier. The next
day the stain was fine. Now the pathologist is saying that the sections
look air dried or cooked. I checked the temperatures on the
waterbaths, embedding stations, vip processor and the stainer. We have
a Shandon Gemini stainer. This is a mixture of GI, Skin,Gyn, and other
biopsies. The surgicals are not affected. The only other thing I can
think of is the coverslipper. We have a Leica automatic glass
coverslipper. Any help in this situation would be greatly appreciated.
> Allison Scott
> Histology Supervisor
> LBJ Hospital
> Houston, Texas
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