[Histonet] RE: H&E Issues

From:"Kari Bradshaw"

What type of paraffin are you using?  We had similar problems awhile
back. The paraffin we had used for many years had a change in where is
was being manufactured, which resulted in somewhat of a different
recipe.  Call me and I can fill you in on what we were using and how we
fixed the problem.

Kari Bradshaw
Anatomic Pathology Manager
Lower Columbia Pathologists
1217 14th Ave
Longview, WA 98632

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott,
Allison D
Sent: Friday, May 11, 2007 9:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: H&E Issues

>  -----Original Message-----
> From: 	Scott, Allison D  
> Sent:	Friday, May 11, 2007 11:31 AM
> To:	'histonet@lists.utsouthwester.edu'
> Subject:	H&E Issues
> We are having a problem with our H&E slides.  The problem just started
this week.  One of our pathologist said that the stain was too dark.
After looking at the solutions in the stainer, I determined that the
bluing solution had not been changed as it should have been and I
changed it.  I also changed the hematoxylin and the clarifier.  The next
day the stain was fine.  Now the pathologist is saying that the sections
look air dried or cooked.  I checked the temperatures on  the
waterbaths, embedding stations, vip processor and the stainer.  We have
a Shandon Gemini stainer.  This is a mixture of GI, Skin,Gyn, and other
biopsies.  The surgicals are not affected.  The only other thing I can
think of is the coverslipper.  We have a Leica automatic glass
coverslipper.  Any help in this situation would be greatly appreciated.
Happy Friday
> Allison Scott
> Histology Supervisor
> LBJ Hospital
> Houston, Texas

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