[Histonet] RE: H&E Issues

From:"Kari Bradshaw"



What type of paraffin are you using?  We had similar problems awhile
back. The paraffin we had used for many years had a change in where is
was being manufactured, which resulted in somewhat of a different
recipe.  Call me and I can fill you in on what we were using and how we
fixed the problem.

Kari Bradshaw
Anatomic Pathology Manager
Lower Columbia Pathologists
1217 14th Ave
Longview, WA 98632
360.425.5620
kbradshaw@lcpath.com
 

-----Original Message-----
From: histonet-bounces@lists.utsouthwestern.edu
[mailto:histonet-bounces@lists.utsouthwestern.edu] On Behalf Of Scott,
Allison D
Sent: Friday, May 11, 2007 9:43 AM
To: histonet@lists.utsouthwestern.edu
Subject: [Histonet] FW: H&E Issues



>  -----Original Message-----
> From: 	Scott, Allison D  
> Sent:	Friday, May 11, 2007 11:31 AM
> To:	'histonet@lists.utsouthwester.edu'
> Subject:	H&E Issues
> 
> We are having a problem with our H&E slides.  The problem just started
this week.  One of our pathologist said that the stain was too dark.
After looking at the solutions in the stainer, I determined that the
bluing solution had not been changed as it should have been and I
changed it.  I also changed the hematoxylin and the clarifier.  The next
day the stain was fine.  Now the pathologist is saying that the sections
look air dried or cooked.  I checked the temperatures on  the
waterbaths, embedding stations, vip processor and the stainer.  We have
a Shandon Gemini stainer.  This is a mixture of GI, Skin,Gyn, and other
biopsies.  The surgicals are not affected.  The only other thing I can
think of is the coverslipper.  We have a Leica automatic glass
coverslipper.  Any help in this situation would be greatly appreciated.
Happy Friday
> 
> Allison Scott
> Histology Supervisor
> LBJ Hospital
> Houston, Texas

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