It is generally recommended that you spin down your fluid, decant, and then
add the fixative and re-spin. Using a 1/2 fluid and 1/2 fixative method
gives you a weak solution for fixation. Whether you use 95% alcohol (ethyl
or reagent) or 10% formalin is a personal choice - it depends on whether
your pathologist likes to see formalin fixed morphology or alcohol fixed
You didn't mention what method you use to hold all those loose cells
together for processing/embedding/sectioning. There are several simple
methods available to make your life easier and give the most cellular
blocks/slides. If you are interested in info about those, just let me know.
Beth Cox, SCT/HT(ASCP)
Schools of Histotechnology
Department of Anatomic Pathology, 100RO
William Beaumont Hospital
3601 W. 13 Mile Road
Royal Oak, MI 48073-6769
Date: Tue, 1 May 2007 12:51:36 -0700
From: "Heckford, Karen - SMMC-SF"
Subject: [Histonet] Cell Button Prep
To: "Histonet (firstname.lastname@example.org)"
Content-Type: text/plain; charset="us-ascii"
I was wondering if anyone would be so kind as giving input on the different
ways of preparing a cell button. We work a lot with Pleural Fluid and
Ascites Fluid. I have been told two different ways. One is just 1/2 and
1/2 with fluid and 95%alcohol and spend down decant and get your cell
button. The other is spend down the fluid decant add 10%formalin respin and
let set then get your cell button. Of course this all after I have made my
cytospins. We do not have a ThinPrep type machine. Any input would be very
appreciative. I realize there is probably more ways to do this that I have
not heard of.
Karen Heckford HT (ASCP) CE
Lead Histology Technician
St. Mary's Medical Center
450 Stanyan St.
San Francisco, Ca. 94117
415-668-1000 ext. 6167
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