[Histonet] FW: H&E Issues

From:"Scott, Allison D"

>  -----Original Message-----
> From: 	Scott, Allison D =20
> Sent:	Friday, May 11, 2007 11:31 AM
> To:	'histonet@lists.utsouthwester.edu'
> Subject:	H&E Issues
> We are having a problem with our H&E slides.  The problem just started this week.  One of our pathologist said that the stain was too dark.  After looking at the solutions in the stainer, I determined that the bluing solution had not been changed as it should have been and I  changed it.  I also changed the hematoxylin and the clarifier.  The next day the stain was fine.  Now the pathologist is saying that the sections look air dried or cooked.  I checked the temperatures on  the waterbaths, embedding stations, vip processor and the stainer.  We have a Shandon Gemini stainer.  This is a mixture of GI, Skin,Gyn, and other biopsies.  The surgicals are not affected.  The only other thing I can think of is the coverslipper.  We have a Leica automatic glass coverslipper.  Any help in this situation would be greatly appreciated.   Happy Friday
> Allison Scott
> Histology Supervisor
> LBJ Hospital
> Houston, Texas

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