> -----Original Message-----
> From: Scott, Allison D =20
> Sent: Friday, May 11, 2007 11:31 AM
> To: 'firstname.lastname@example.org'
> Subject: H&E Issues
> We are having a problem with our H&E slides. The problem just started this week. One of our pathologist said that the stain was too dark. After looking at the solutions in the stainer, I determined that the bluing solution had not been changed as it should have been and I changed it. I also changed the hematoxylin and the clarifier. The next day the stain was fine. Now the pathologist is saying that the sections look air dried or cooked. I checked the temperatures on the waterbaths, embedding stations, vip processor and the stainer. We have a Shandon Gemini stainer. This is a mixture of GI, Skin,Gyn, and other biopsies. The surgicals are not affected. The only other thing I can think of is the coverslipper. We have a Leica automatic glass coverslipper. Any help in this situation would be greatly appreciated. Happy Friday
> Allison Scott
> Histology Supervisor
> LBJ Hospital
> Houston, Texas
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