Dear all,I would like to perform Alizarin Red staining for mineralization nodules in primary osteoblasts, Does anyone have a protocol about it?=0AIn addition, for mineralization nodule formation, we should add ascorbic acid and beta-glycerolphosphate into the culture medium, my question is :  use which solution to dissovle these drugs (distilled water, or culture medium).Thank you. Guofeng      ___________________________________________________________ ÇÀ×¢ÑÅ»¢Ãâ·Ñ=D3ÊÏä3.5GÈÝÁ¿£¬20M¸½¼þ£¡ http://cn.mail.yahoo.com
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