Re: [Histonet] section thickness

From:"Anila Syed"

Hello,

we always get best results with 5um

Best wishes

Anila Syed
>
> ----- Original Message ----- 
> From: "Edwards, R.E." 
> To: 
> Sent: Thursday, May 18, 2006 6:43 AM
> Subject: [Histonet] section thickness
>
>
>
>
>
> In your  invaluable, but,  no  doubt differing opinions, what  is  the
> optimal section thickness   for formalin fixed paraffin processed tissues
> for  immunostaining, using  DAB  as  chromogen?, there has been  some
> discussion here  whether  it  should be 4,5,6, or  7microns, many thanks.
>
>
>                    Richard  Edwards
>
>
>                     MRC TOXICOLOGY UNIT
>
>                              LEICESTER..U.K.......
>
>
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