Re: [Histonet] floaters

From:Rene J Buesa

  First try to find the source of the floater.
  Compare the stained slide containing the floater with the block surface. If the tissue seen as floater is not in the block, it was picked up in the water bath. The solution is to clean the surface of the water with a tissue after each block is cut.
  If you can identify in the block the source of the floater it means that contamination occurred either during cassetting or during embedding.
  Make sure that the cassetting is done in a way that prevents carry-on of small pieces of tissue. The embedding center (the heating wells for the forceps) have to be empied with a pipette once embedding is finished. After that use a Q-tip to completely dry-clean the wells. Forceps should be cleaned after each block is embedded.
  Care is the solution and attention to detail is the only solution.
  Hope this will help you.
  René J.

"Webb, Dorothy L"  wrote:
  How does everyone handle their embedding techniques in regard to
contaminating one tissue with another? We are doing here what I have
done at the other lab I worked at and lately the pathologist is seeing a
few "floaters" on a slide where they shouldn't be!! Just thought I
would like to throw this out for discussion and hopefully come away with
a technique better than ours!! Thanks, as always!!!
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