Re: [Histonet] DNA/RNA Extraction Kits for paraffinised tissues

From:"louise renton"

Dear Shirley,

My information may be somewhat out of date as it is several years
since I did DNA extration on formalin fixed /praffin embedded tissues,
but at that time I found that the commercial kits (specifically the
ones using spin columns) were not as good as the old fashioned method
of phenol chloroform.

The amount/quality of DNA available is directly related to the type
and length of fixation so harsh unbuffered fixation will degrade the

This is my 2 cents worth

On 5/23/06, Shirley PHUA  wrote:
> Dear All
> Have anyone heard of any DNA/RNA extraction kits for formalin-fixed,
> paraffinised tissues? I will greatly appreciate if you can share some of
> your feedbacks. Were your yields good?
> Thanks in advance!
> Shirley Phua
> Histopathology Laboratory
> Centre for Forensic Medicine
> Health Sciences Authority
> DID : 62130654
> _______________________________________________
> Histonet mailing list

Louise Renton
Bone Research Unit
University of the Witwatersrand
South Africa
"There are nights when the wolves are silent and only the moon howls".
George Carlin

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